细胞内固态核磁共振：一种用于研究生物膜的新兴技术。
In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes.
作者信息
Warnet Xavier L, Arnold Alexandre A, Marcotte Isabelle, Warschawski Dror E
机构信息
UMR 7099, CNRS-Université Paris Diderot, Institut de Biologie Physico-Chimique, Paris, France.
Department of Chemistry, Université du Québec à Montréal, Montréal, Québec, Canada.
出版信息
Biophys J. 2015 Dec 15;109(12):2461-2466. doi: 10.1016/j.bpj.2015.10.041.
Abstract
Biological molecular processes are often studied in model systems, which simplifies their inherent complexity but may cause investigators to lose sight of the effects of the molecular environment. Information obtained in this way must therefore be validated by experiments in the cell. NMR has been used to study biological cells since the early days of its development. The first NMR structural studies of a protein inside a cell (by solution-state NMR) and of a membrane protein (by solid-state NMR) were published in 2001 and 2011, respectively. More recently, dynamic nuclear polarization, which has been used to enhance the signal in solid-state NMR, has also been applied to the study of frozen cells. Much progress has been made in the past 5 years, and in this review we take stock of this new technique, which is particularly appropriate for the study of biological membranes.
摘要
生物分子过程通常在模型系统中进行研究，这简化了其固有的复杂性，但可能导致研究人员忽视分子环境的影响。因此，通过这种方式获得的信息必须通过细胞实验进行验证。自NMR发展初期以来，它就一直被用于研究生物细胞。细胞内蛋白质的首次NMR结构研究（通过溶液态NMR）和膜蛋白的首次NMR结构研究（通过固态NMR）分别于2001年和2011年发表。最近，用于增强固态NMR信号的动态核极化也已应用于冷冻细胞的研究。在过去5年里已经取得了很大进展，在本综述中，我们对这项特别适用于生物膜研究的新技术进行了评估。
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