Hou Yu-Chen, Wu Jin-Ming, Wang Ming-Yang, Wu Ming-Hsun, Chen Kuen-Yuan, Yeh Sung-Ling, Lin Ming-Tsan
Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan ; School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan.
Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan.
Mediators Inflamm. 2015;2015:826319. doi: 10.1155/2015/826319. Epub 2015 Nov 26.
This study evaluated the impact of different doses of Astragalus polysaccharides (APS) on the functional status and phenotype of T cells during polymicrobial sepsis.
On day 1 after cecal ligation and puncture, mice were treated with either saline, 100 (A100), 200 (A200), or 400 mg APS/kg body weight (BW) (A400) by an intraperitoneal injection daily for 4 days. All mice were sacrificed 5 days after the operation.
APS treatment reversed the sepsis-induced decrement in the T helper (Th) cell population, and the percentage of activated Th cells also increased in the spleen and Peyer's patches. APS administration downregulated the percentages of circulating Th2 cells and regulatory T cells (Treg), and the percentage of Th17 cells in blood was upregulated in the A400 group. Weight loss and kidney injury were attenuated in the A100 and A200 groups but not in the A400 group at the end of the study.
Treatments with 100 and 200 mg APS/kg BW reduced Treg populations and elicited a more-balanced Th1/Th2 response that consequently attenuated immunosuppression in polymicrobial sepsis. High-dose APS administration led to excessive responses of Th17 cells which may have adverse effects in sepsis-induced organ injury.
本研究评估了不同剂量黄芪多糖（APS）对多微生物脓毒症期间T细胞功能状态和表型的影响。
在盲肠结扎和穿刺术后第1天，小鼠每天腹腔注射生理盐水、100（A100）、200（A200）或400 mg APS/千克体重（BW）（A400），持续4天。所有小鼠在术后5天处死。
APS治疗逆转了脓毒症诱导的辅助性T（Th）细胞数量减少，脾脏和派尔集合淋巴结中活化Th细胞的百分比也增加。APS给药下调了循环中Th2细胞和调节性T细胞（Treg）的百分比，A400组血液中Th1细胞的百分比上调。在研究结束时，A100和A200组体重减轻和肾损伤减轻，但A400组未减轻。
100和200 mg APS/千克BW治疗可减少Treg细胞数量，并引发更平衡的Th1/Th2反应，从而减轻多微生物脓毒症中的免疫抑制。高剂量APS给药导致Th1细胞过度反应，这可能对脓毒症诱导的器官损伤产生不利影响。
