对古夷苏木属（豆科，云实亚科）的微卫星开发揭示了二倍体和多倍体物种。
Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species.
作者信息
Tosso Felicien, Doucet Jean-Louis, Kaymak Esra, Daïnou Kasso, Duminil Jérôme, Hardy Olivier J
机构信息
Tropical Forestry, Management of Forest Resources, TERRA, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium.
Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.
出版信息
Appl Plant Sci. 2016 Jul 13;4(7). doi: 10.3732/apps.1600029. eCollection 2016 Jul.
PREMISE OF THE STUDY
Nuclear microsatellites (nSSRs) were designed for Guibourtia tessmannii (Fabaceae, Caesalpinioideae), a highly exploited African timber tree, to study population genetic structure and gene flow.
METHODS AND RESULTS
We developed 16 polymorphic nSSRs from a genomic library tested in three populations of G. tessmannii and two populations of G. coleosperma. These nSSRs display three to 14 alleles per locus (mean 8.94) in G. tessmannii. Cross-amplification tests in nine congeneric species demonstrated that the genus Guibourtia contains diploid and polyploid species. Flow cytometry results combined with nSSR profiles suggest that G. tessmannii is octoploid.
CONCLUSIONS
nSSRs revealed that African Guibourtia species include both diploid and polyploid species. These markers will provide information on the mating system, patterns of gene flow, and genetic structure of African Guibourtia species.
研究前提
为高度开发利用的非洲木材树种特斯曼古夷苏木（豆科，云实亚科）设计了核微卫星（nSSRs），以研究其种群遗传结构和基因流。
方法与结果
我们从一个基因组文库中开发了16个多态性nSSRs，并在特斯曼古夷苏木的三个种群和鞘籽古夷苏木的两个种群中进行了测试。这些nSSRs在特斯曼古夷苏木中每个位点显示3至14个等位基因（平均8.94个）。在9个同属物种中的交叉扩增测试表明，古夷苏木属包含二倍体和多倍体物种。流式细胞术结果与nSSR图谱相结合表明，特斯曼古夷苏木是八倍体。
结论
nSSRs显示非洲古夷苏木属物种包括二倍体和多倍体物种。这些标记将提供有关非洲古夷苏木属物种交配系统、基因流模式和遗传结构的信息。
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