与癌症和衰老相关的内源性DNA损伤。
Endogenous DNA damage as related to cancer and aging.
作者信息
Ames B N
机构信息
Department of Biochemistry, University of California, Berkeley 94720.
出版信息
Mutat Res. 1989 Sep;214(1):41-6. doi: 10.1016/0027-5107(89)90196-6.
The endogenous background level of oxidant-induced DNA damage in vivo has been assayed by measuring 8-hydroxydeoxyguanosine (oh8dG), thymine glycol and thymidine glycol in urine and oh8dG in DNA. The level of oxidative DNA damage as measured by oh8dG in normal rat liver is shown to be extensive (1/130,000 bases in nuclear DNA and 1/8000 bases in mitochondrial DNA), especially in mtDNA. The methylation adduct 7-methylguanine (m7G) has also been found. m7G is one of about 5 adducts found on methylating DNA, and oh8dG is one of about 20 adducts found on oxidizing DNA, e.g., by radiation. We also discuss 3 hitherto unrecognized antioxidants in man.
通过测量尿液中的8-羟基脱氧鸟苷（oh8dG）、胸腺嘧啶乙二醇和胸苷乙二醇以及DNA中的oh8dG，已对体内氧化剂诱导的DNA损伤的内源性背景水平进行了测定。通过oh8dG测定的正常大鼠肝脏中氧化性DNA损伤水平显示很高（核DNA中为1/130,000个碱基，线粒体DNA中为1/8000个碱基），尤其是在线粒体DNA中。还发现了甲基化加合物7-甲基鸟嘌呤（m7G）。m7G是在DNA甲基化时发现的约5种加合物之一，而oh8dG是在DNA氧化（例如通过辐射）时发现的约20种加合物之一。我们还讨论了人类中迄今未被认识的3种抗氧化剂。
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