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采用Etest法对从口腔念珠菌病患者中分离出的念珠菌进行药敏试验。

Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis.

作者信息

Song You Bum, Suh Moo Kyu, Ha Gyoung Yim, Kim Heesoo

机构信息

Department of Dermatology, Dongguk University College of Medicine, Gyeongju, Korea.

Department of Laboratory Medicine, Dongguk University College of Medicine, Gyeongju, Korea.

出版信息

Ann Dermatol. 2015 Dec;27(6):715-20. doi: 10.5021/ad.2015.27.6.715. Epub 2015 Dec 7.

DOI:10.5021/ad.2015.27.6.715
PMID:26719641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4695424/
Abstract

BACKGROUND

The necessity of performing antifungal susceptibility tests is recently increasing because of frequent cases of oral candidiasis caused by antifungal-resistant Candida species. The Etest (BioMerieux, Marcy l'Etoile, France) is a rapid and easy-to-perform in vitro antifungal susceptibility test.

OBJECTIVE

The purpose of this study was to determine the minimal inhibitory concentrations (MICs) of antifungal agents by using the Etest for Candida species isolated from patients with oral candidiasis.

METHODS

Forty-seven clinical isolates of Candida species (39 isolates of Candida albicans, 5 isolates of C. glabrata, and 3 isolates of C. tropicalis) were tested along with a reference strain (C. albicans ATCC 90028). The MIC end points of the Etest for fluconazole, itraconazole, voriconazole, and amphotericin B susceptibility were read after the 24-hour incubation of each isolate on RPMI 1640 agar.

RESULTS

All Candida isolates were found susceptible to voriconazole and amphotericin B. However, all five isolates of C. glabrata were resistant to itraconazole, among which two isolates were also resistant to fluconazole.

CONCLUSION

This study revealed that the Etest represented a simple and efficacious method for antifungal susceptibility testing of Candida species isolated from oral candidiasis patients. Therefore, voriconazole and amphotericin B should be recommended as effective alternatives for the treatment of oral candidiasis.

摘要

背景

由于耐抗真菌药念珠菌属引起的口腔念珠菌病病例频发，进行抗真菌药敏试验的必要性近来不断增加。Etest（法国马赛 - 埃托瓦勒生物梅里埃公司）是一种快速且易于操作的体外抗真菌药敏试验。

目的

本研究的目的是通过使用Etest测定从口腔念珠菌病患者分离出的念珠菌属对抗真菌药物的最低抑菌浓度（MIC）。

方法

对47株念珠菌临床分离株（39株白色念珠菌、5株光滑念珠菌和3株热带念珠菌）以及一株参考菌株（白色念珠菌ATCC 90028）进行检测。将各分离株在RPMI 1640琼脂上孵育24小时后，读取Etest针对氟康唑、伊曲康唑、伏立康唑和两性霉素B药敏试验的MIC终点值。

结果

所有念珠菌分离株均对伏立康唑和两性霉素B敏感。然而，所有5株光滑念珠菌分离株均对伊曲康唑耐药，其中2株对氟康唑也耐药。

结论

本研究表明，Etest是一种用于从口腔念珠菌病患者分离出的念珠菌属进行抗真菌药敏试验的简单且有效的方法。因此，伏立康唑和两性霉素B应被推荐为治疗口腔念珠菌病的有效替代药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3c/4695424/2fbdc074bf14/ad-27-715-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3c/4695424/2fbdc074bf14/ad-27-715-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b3c/4695424/2fbdc074bf14/ad-27-715-g001.jpg

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