Hsiung G D
Virology Reference Laboratory, Veterans Administration Medical Center, West Haven, Connecticut 06516.
Yale J Biol Med. 1989 Mar-Apr;62(2):79-88.
The contribution of cell culture systems in the diagnosis of viral infections has been well recognized over the years. Not only did such systems make possible the direct isolation and identification of viruses, but also the production of viral diagnostic reagents for rapid diagnosis, the evaluation of antiviral agents, and the production of vaccines for the control of viral diseases. Although many reagents for rapid detection of viral antigens/genomes are currently available, none will make possible discoveries of new viral agents. Thus sensitive cell culture systems are still essential for the rapid and accurate diagnosis of viral infections. Since, as yet, no single cell culture system is susceptible to all viruses, the constant search for additional sensitive cell culture systems for detecting those unknown and/or currently non-cultivable viral agents continues to be an open area of investigation in the field of diagnostic virology.
多年来，细胞培养系统在病毒感染诊断中的作用已得到充分认可。这类系统不仅使病毒的直接分离和鉴定成为可能，还能生产用于快速诊断的病毒诊断试剂、评估抗病毒药物以及生产用于控制病毒性疾病的疫苗。尽管目前有许多用于快速检测病毒抗原/基因组的试剂，但没有一种能发现新的病毒病原体。因此，灵敏的细胞培养系统对于病毒感染的快速准确诊断仍然至关重要。由于目前尚无单一的细胞培养系统能对所有病毒敏感，持续寻找用于检测那些未知和/或目前无法培养的病毒病原体的其他灵敏细胞培养系统仍是诊断病毒学领域一个有待开拓的研究方向。
