Duque Ana Paula do Nascimento, Pinto Nícolas de Castro Campos, Mendes Renata de Freitas, da Silva Josiane Mello, Aragão Danielle Maria de Oliveira, Castañon Maria Christina Marques Nogueira, Scio Elita
Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Department of Morphology, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
J Pharm Pharmacol. 2016 Jan;68(1):128-38. doi: 10.1111/jphp.12496. Epub 2016 Jan 4.
To evaluate the in-vivo wound healing and in-vitro antioxidant activity of gels containing the ethyl acetate extract of Cecropia pachystachya (ECP) 2% and 5%, and to perform the chemical fingerprint of ECP by HPLC-DAD.
The wound healing activity of the gels was evaluated for 21 days, using the excision model in rats followed by histopathological and histomorphometric analysis. The in-vitro antioxidant effect of ECP was investigated using 1-diphenyl-2-picrylhydrazyl, reducing power, β-carotene bleaching and thiobarbituric acid reactive substances assays. Also, a HPLC analysis was performed to identify the chemical markers orientin, iso-orientin and chlorogenic acid.
The group of animals treated with ECP 5% presented oedema and inflammatory infiltrate with less intensity than the other groups. Both ECP 2% and 5% gels showed less neovascularization and cellularity, and better tissue repair when compared to the control, which showed a younger and homogeneous tissue.
This study had demonstrated that the ECP gels promoted the acceleration of the healing process when compared to the control group. Wound contraction, angiogenesis, epithelialization and the collagen deposition support further evaluation of C. pachystachya leaves in the topical treatment and management of skin wounds.
评估含2%和5%美洲合欢（Cecropia pachystachya）乙酸乙酯提取物（ECP）的凝胶在体内的伤口愈合情况和体外抗氧化活性，并通过高效液相色谱-二极管阵列检测法（HPLC-DAD）对ECP进行化学指纹图谱分析。
采用大鼠切除模型，通过组织病理学和组织形态计量学分析，对凝胶的伤口愈合活性进行21天的评估。采用1,1-二苯基-2-苦基肼自由基（DPPH）、还原力、β-胡萝卜素漂白和硫代巴比妥酸反应性物质（TBARS）测定法研究ECP的体外抗氧化作用。此外，进行HPLC分析以鉴定化学标志物荭草素、异荭草素和绿原酸。
用5% ECP处理的动物组出现的水肿和炎性浸润强度低于其他组。与对照组相比，2%和5% ECP凝胶均显示出较少的新生血管形成和细胞数量，以及更好的组织修复，对照组显示出更年轻且均匀的组织。
本研究表明，与对照组相比，ECP凝胶促进了愈合过程的加速。伤口收缩、血管生成、上皮形成和胶原蛋白沉积支持对美洲合欢叶在皮肤伤口局部治疗和管理中的进一步评估。
