Ersel Murat, Uyanikgil Yigit, Karbek Akarca Funda, Ozcete Enver, Altunci Yusuf Ali, Karabey Fatih, Cavusoglu Turker, Meral Ayfer, Yigitturk Gurkan, Oyku Cetin Emel
Department of Emergency Medicine, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
Department of Histology and Embryology, Ege University, Faculty of Medicine, Bornova, Izmir, Turkey.
Med Sci Monit. 2016 Apr 1;22:1064-78. doi: 10.12659/msm.897981.
The wound healing process is complex and still poorly understood. Sericin is a silk protein synthesized by silk worms (Bombyx mori). The objective of this study was to evaluate in vivo wound healing effects of a sericin-containing gel formulation in an incision wound model in rats.
MATERIAL/METHODS: Twenty-eight Wistar-Albino rats were divided into 4 groups (n=7). No intervention or treatment was applied to the Intact control group. For other groups, a dorsal skin flap (9×3 cm) was drawn and pulled up with sharp dissection. The Sham operated group received no treatment. The Placebo group received placebo gel without sericin applied to the incision area once a day from day 0 to day 9. The Sericin Group 3 received 1% sericin gel applied to the incision area once a day from day 0 to day 9. Hematoxylin and eosin stain was applied for histological analysis and Mallory-Azan staining was applied for histoimmunochemical analysis of antibodies and iNOS (inducible nitric oxide synthase), and desmin was applied to paraffin sections of skin wound specimens. Parameters of oxidative stress were measured in the wound area.
Epidermal thickness and vascularization were increased, and hair root degeneration, edema, cellular infiltration, collagen discoloration, and necrosis were decreased in Sericin group in comparison to the Placebo group and the Sham operated group. Malonyldialdehyde (MDA) levels were decreased, but superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were increased in the sericin group.
We found that sericin had significant positive effects on wound healing and antioxidant activity. Sericin-based formulations can improve healing of incision wounds.
伤口愈合过程复杂，目前仍知之甚少。丝胶蛋白是家蚕（Bombyx mori）合成的一种丝蛋白。本研究的目的是在大鼠切口伤口模型中评估含丝胶蛋白凝胶制剂的体内伤口愈合效果。
材料/方法：将28只Wistar白化大鼠分为4组（n = 7）。完整对照组不进行任何干预或治疗。对于其他组，绘制一个背部皮瓣（9×3 cm），并用锐性解剖将其掀起。假手术组不接受治疗。安慰剂组从第0天到第9天每天在切口区域涂抹不含丝胶蛋白的安慰剂凝胶。丝胶蛋白组3从第0天到第9天每天在切口区域涂抹1%丝胶蛋白凝胶。应用苏木精和伊红染色进行组织学分析，应用马洛里-阿赞染色进行抗体和诱导型一氧化氮合酶（iNOS）的组织免疫化学分析，应用结蛋白对皮肤伤口标本的石蜡切片进行分析。在伤口区域测量氧化应激参数。
与安慰剂组和假手术组相比，丝胶蛋白组的表皮厚度和血管化增加，毛根退变、水肿、细胞浸润、胶原变色和坏死减少。丝胶蛋白组的丙二醛（MDA）水平降低，但超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性增加。
我们发现丝胶蛋白对伤口愈合和抗氧化活性有显著的积极影响。基于丝胶蛋白的制剂可以改善切口伤口的愈合。
