Desoubeaux Guillaume, Cabanne Églantine, Franck-Martel Claire, Gombert Martin, Gyan Emmanuel, Lissandre Séverine, Renaud Marc, Monjanel Hélène, Dartigeas Caroline, Bailly Éric, Van Langendonck Nathalie, Chandenier Jacques
Service de Parasitologie-Mycologie-Médecine tropicale, CHU de Tours, Tours, France Faculté de Médecine, Université François-Rabelais, CEPR-INSERM U1100/Équipe 3, Université François-Rabelais Tours, France.
Service de Parasitologie-Mycologie-Médecine tropicale, CHU de Tours, Tours, France.
J Clin Pathol. 2016 Aug;69(8):726-30. doi: 10.1136/jclinpath-2015-203385. Epub 2016 Jan 4.
Pulmonary toxoplasmosis has become a very rare parasitic infection since the advent of highly active antiretroviral therapies. It is generally diagnosed by the direct microscopic observation of Toxoplasma gondii tachyzoites in bronchoalveolar lavage fluid (BALF). The aim of this study was to assess possible improvements in diagnostic performance associated with the use of real-time PCR.
This prospective study was carried out on BALFs obtained from immunocompromised patients over a 2-year period. We systematically compared the results of conventional staining with those of molecular detection.
Two cases of pulmonary toxoplasmosis were diagnosed for a total of 336 samples. PCR did not detect any additional cases and was more time-consuming than conventional staining.
Conventional staining is a reliable technique and is probably the most appropriate method for experienced microbiology laboratories, whereas T. gondii-specific PCR may be useful for laboratories with less experience in parasitology.
2015_030, May 27th 2015.
自从高效抗逆转录病毒疗法问世以来，肺弓形虫病已成为一种非常罕见的寄生虫感染。它通常通过在支气管肺泡灌洗（BALF）液中直接显微镜观察刚地弓形虫速殖子来诊断。本研究的目的是评估与使用实时聚合酶链反应（PCR）相关的诊断性能可能的改善情况。
这项前瞻性研究是对2年内从免疫功能低下患者获得的BALF进行的。我们系统地比较了传统染色结果与分子检测结果。
在总共336份样本中诊断出2例肺弓形虫病。PCR未检测到任何其他病例，并且比传统染色更耗时。
传统染色是一种可靠的技术，可能是经验丰富的微生物实验室最合适的方法，而针对弓形虫的PCR可能对寄生虫学经验较少的实验室有用。
2015_030，2015年5月27日。
