Rendeková Katarína, Fialová Silvia, Jánošová Lucia, Mučaji Pavel, Slobodníková Lívia
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia.
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia.
Molecules. 2015 Dec 30;21(1):E50. doi: 10.3390/molecules21010050.
The purpose of this study was to detect the effectiveness of Cotinus coggygria Scop. leaves methanol extract against planktonic and biofilm growth forms of Staphylococcus aureus. The antimicrobial activity was determined by the broth microdilution test. Minimal inhibitory concentrations and minimal bactericidal concentrations were detected against two collection and ten clinical S. aureus strains. Anti-biofilm activity of the tested extract was detected using 24 h bacterial biofilm on the surface of microtiter plate wells. The biofilm inhibitory activity was evaluated visually after 24 h interaction of extract with biofilm, and the eradicating activity by a regrowth method. The tested extract showed bactericidal activity against all S. aureus strains (methicillin susceptible or methicillin resistant) in concentrations ranging from 0.313 to 0.625 mg·mL(-1). Biofilm inhibitory concentrations were 10-times higher and biofilm eradicating concentrations 100-times higher (8 and 32 mg·mL(-1), respectively). The phytochemical analysis of C. coggygria leaves 60% methanol extract performed by LC-DAD-MS/MS revealed quercetin rhamnoside, methyl gallate, and methyl trigallate as main constituents. Results of our study indicate that C. coggygria, rich in tannins and flavonoids, seems to be a prospective topical antibacterial agent with anti-biofilm activity.
本研究的目的是检测黄栌叶甲醇提取物对金黄色葡萄球菌浮游菌和生物膜生长形式的有效性。通过肉汤微量稀释试验测定抗菌活性。检测了该提取物对两株收集的金黄色葡萄球菌菌株和十株临床金黄色葡萄球菌菌株的最低抑菌浓度和最低杀菌浓度。使用微量滴定板孔表面24小时形成的细菌生物膜检测受试提取物的抗生物膜活性。在提取物与生物膜相互作用24小时后，通过肉眼评估生物膜抑制活性，并通过再生长法评估根除活性。受试提取物对所有金黄色葡萄球菌菌株（甲氧西林敏感或甲氧西林耐药）均表现出杀菌活性，浓度范围为0.313至0.625 mg·mL(-1)。生物膜抑制浓度高10倍，生物膜根除浓度高100倍（分别为8和32 mg·mL(-1)）。通过LC-DAD-MS/MS对黄栌叶60%甲醇提取物进行的植物化学分析显示，主要成分有槲皮素鼠李糖苷、没食子酸甲酯和三没食子酸甲酯。我们的研究结果表明，富含单宁和黄酮类化合物的黄栌似乎是一种具有抗生物膜活性的潜在局部抗菌剂。
