Bernard Clément, Juin Camille, Vitry Marine, Le Van Thanh Danh, Verdon Julien, Toullec Anne-Solène, Imbert Christine, Girardot Marion
Laboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, F-86073 Poitiers, France.
Pharmaceuticals (Basel). 2020 Dec 18;13(12):477. doi: 10.3390/ph13120477.
is an opportunistic pathogen involved in many infections, especially linked to implanted medical devices. Its ability to form biofilms complicates the treatment of these infections as few molecules are active against sessile . The aim of this study was to evaluate the potential of leaves, three-month-old and one-year-old stems of L. against biofilm growth. Extractions with a polarity gradient were carried out on hydroacetonic extracts and followed by fractionation steps. The obtained extracts and fractions were tested for their anti-biofilm growth activity against using XTT method. Compounds of active subfractions were identified by LC-MS. The hexane extracts from leaves and stems were the most active against the fungus with IC at 500 and 250 µg/mL. Their bioguided fractionation led to 4 subfractions with IC between 62.5 and 125 µg/mL. Most of the components identified in active subfractions were fatty acids and terpenoïds.
是一种涉及多种感染的机会致病菌，尤其与植入式医疗设备相关。其形成生物膜的能力使这些感染的治疗变得复杂，因为很少有分子对固着菌有活性。本研究的目的是评估L.的叶片、三个月大和一岁茎对生物膜生长的潜在作用。对氢丙酮提取物进行极性梯度萃取，然后进行分馏步骤。使用XTT方法测试所得提取物和馏分对生物膜生长的抗生物膜活性。通过LC-MS鉴定活性亚组分的化合物。叶片和茎的己烷提取物对真菌活性最强，IC50分别为500和250 µg/mL。它们的生物导向分馏产生了4个亚组分，IC50在62.5至125 µg/mL之间。在活性亚组分中鉴定出的大多数成分是脂肪酸和萜类化合物。
