使用BioGRID数据库分析酵母蛋白质和遗传相互作用。
Use of the BioGRID Database for Analysis of Yeast Protein and Genetic Interactions.
作者信息
Oughtred Rose, Chatr-aryamontri Andrew, Breitkreutz Bobby-Joe, Chang Christie S, Rust Jennifer M, Theesfeld Chandra L, Heinicke Sven, Breitkreutz Ashton, Chen Daici, Hirschman Jodi, Kolas Nadine, Livstone Michael S, Nixon Julie, O'Donnell Lara, Ramage Lindsay, Winter Andrew, Reguly Teresa, Sellam Adnane, Stark Chris, Boucher Lorrie, Dolinski Kara, Tyers Mike
机构信息
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544;
Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, Québec H3C 3J7, Canada;
出版信息
Cold Spring Harb Protoc. 2016 Jan 4;2016(1):pdb.prot088880. doi: 10.1101/pdb.prot088880.
Abstract
The BioGRID database is an extensive repository of curated genetic and protein interactions for the budding yeast Saccharomyces cerevisiae, the fission yeast Schizosaccharomyces pombe, and the yeast Candida albicans SC5314, as well as for several other model organisms and humans. This protocol describes how to use the BioGRID website to query genetic or protein interactions for any gene of interest, how to visualize the associated interactions using an embedded interactive network viewer, and how to download data files for either selected interactions or the entire BioGRID interaction data set.
摘要
BioGRID数据库是一个广泛的数据库，其中精心整理了酿酒酵母、粟酒裂殖酵母、白色念珠菌SC5314以及其他几种模式生物和人类的遗传与蛋白质相互作用信息。本方案描述了如何使用BioGRID网站查询任何感兴趣基因的遗传或蛋白质相互作用，如何使用嵌入式交互式网络查看器可视化相关相互作用，以及如何下载选定相互作用或整个BioGRID相互作用数据集的数据文件。
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