基质金属蛋白酶2和9在犬猫骨肉瘤中的分布及活性水平
Distribution and activity levels of matrix metalloproteinase 2 and 9 in canine and feline osteosarcoma.
作者信息
Gebhard Christiane, Fuchs-Baumgartinger Andrea, Razzazi-Fazeli Ebrahim, Miller Ingrid, Walter Ingrid
机构信息
Institute of Anatomy, Histology and Embryology (Gebhard, Walter), Institute of Pathology and Forensic Veterinary Medicine (Fuchs-Baumgartinger), VetCore Facility for Research (Razzazi-Fazeli), and Institute of Medical Biochemistry (Miller), University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
出版信息
Can J Vet Res. 2016 Jan;80(1):66-73.
Overexpression of matrix metalloproteinases (MMPs) has been associated with increased tumor aggressiveness and metastasis dissemination. We investigated whether the contrasting metastatic behavior of feline and canine osteosarcoma is related to levels and activities of MMP2 and MMP9. Zymography and immunohistochemistry were used to determine expression levels of MMP2 and MMP9 in canine and feline osteosarcoma. Using immunohistochemistry, increased MMP9 levels were identified in most canine osteosarcomas, whereas cat samples more often displayed moderate levels. High levels of pro-MMP9, pro-MMP2, and active MMP2 were detected by gelatin zymography in both species, with significantly higher values for active MMP2 in canine osteosarcoma. These findings indicate that MMP2 is probably involved in canine and feline osteosarcoma and their expression and activity could be associated with the different metastatic behavior of canine and feline osteosarcoma.
基质金属蛋白酶（MMPs）的过表达与肿瘤侵袭性增加和转移扩散有关。我们研究了猫和犬骨肉瘤不同的转移行为是否与MMP2和MMP9的水平及活性有关。采用酶谱法和免疫组织化学法测定犬和猫骨肉瘤中MMP2和MMP9的表达水平。通过免疫组织化学发现，大多数犬骨肉瘤中MMP9水平升高，而猫的样本中MMP9水平多为中等。通过明胶酶谱法在两个物种中均检测到高水平的前MMP9、前MMP2和活性MMP2，犬骨肉瘤中活性MMP2的值显著更高。这些发现表明，MMP2可能参与了犬和猫骨肉瘤的发生，其表达和活性可能与犬和猫骨肉瘤不同的转移行为有关。
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