Albuquerque Luciana, Kowalewicz-Kulbat Magdalena, Drzewiecka Dominika, Stączek Paweł, d'Auria Giuseppe, Rosselló-Móra Ramon, da Costa Milton S
Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Syst Appl Microbiol. 2016 Mar;39(2):100-5. doi: 10.1016/j.syapm.2015.12.004. Epub 2015 Dec 18.
Two halophilic archaea, designated strains WSM-64(T) and WSM-66, were isolated from a sample taken from a borehole in the currently unexploited Barycz mining area belonging to the "Wieliczka" Salt Mine Company, in Poland. Strains are red pigmented and form non-motile cocci that stain Gram-negative. Strains WSM-64(T) and WSM-66 showed optimum growth at 40 °C, in 20% NaCl and at pH 6.5-7.5. The strains were facultative anaerobes. The major polar lipids of the two strains were phosphatidylglycerol (PG2), phosphatidylglycerol phosphate methyl ester (PGP-Me) and sulfated diglycosyl diether (S-DGD). Menaquinone MK-8 was the major respiratory quinone. The DNA G+C content of strain WSM-64(T) was 61.2 mol% by HPLC method; 61.0 mol% by genome sequencing. Analysis of the almost complete 16S rRNA gene sequence indicated that the strains WSM-64(T) and WSM-66 (99.7% identity) represented a member of the genus Halorhabdus in the family Halobacteriaceae. Both strains formed a distinct cluster and were most closely related to Halorhabdus tiamatea SARL4B(T) and Halorhabdus utahensis AX-2(T) (DSM 12940(T)) (95.4% and 95.6%, respectively). ANI values of WSM-64(T) with the closest relative type strains were <78.5%. Based on 16S rRNA gene sequence and whole genome analyses, physiological and biochemical characteristics we describe a new species represented by strain WSM-64(T) (=DSM 29498(T) =CECT 8673(T)) for which we propose the name Halorhabdus rudnickae sp. nov.
从波兰“维利奇卡”盐矿公司目前尚未开发的巴里奇矿区的一个钻孔采集的样本中，分离出了两株嗜盐古菌，分别命名为菌株WSM-64(T)和WSM-66。这些菌株呈红色色素，形成革兰氏阴性的无运动性球菌。菌株WSM-64(T)和WSM-66在40°C、20% NaCl以及pH 6.5 - 7.5条件下生长最佳。这些菌株为兼性厌氧菌。这两株菌株的主要极性脂类为磷脂酰甘油（PG2）、磷脂酰甘油磷酸甲酯（PGP-Me）和硫酸化二糖基二醚（S-DGD）。甲基萘醌MK-8是主要的呼吸醌。通过高效液相色谱法测定，菌株WSM-64(T)的DNA G+C含量为61.2 mol%；通过基因组测序为61.0 mol%。对几乎完整的16S rRNA基因序列的分析表明，菌株WSM-64(T)和WSM-66（同一性为99.7%）代表盐杆菌科盐红菌属的一个成员。这两株菌株形成了一个独特的聚类，与盐红菌蒂亚马特亚种SARL4B(T)和犹他盐红菌AX-2(T)（DSM 12940(T)）关系最为密切（分别为95.4%和95.6%）。WSM-64(T)与最接近的相关模式菌株的ANI值<78.5%。基于16S rRNA基因序列和全基因组分析、生理生化特征，我们描述了一个以菌株WSM-64(T)（=DSM 29498(T) =CECT 8673(T)）为代表的新物种，我们将其命名为鲁德尼克盐红菌（Halorhabdus rudnickae sp. nov.）。
