Antunes André, Taborda Marco, Huber Robert, Moissl Christine, Nobre M Fernanda, da Costa Milton S
Laboratório de Microbiologia, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):215-20. doi: 10.1099/ijs.0.65316-0.
An extremely halophilic archaeon was isolated from a sample of the brine-sediment interface of the Shaban Deep in the northern Red Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to Halorhabdus utahensis (99.3%), the sole species of the genus Halorhabdus. Strain SARL4B(T) formed non-pigmented colonies and showed optimum growth at 45 degrees C, in 27% (w/v) NaCl and at pH 6.5-7.0. This organism utilized a few complex substrates, such as yeast extract and starch, for growth. Strain SARL4B(T) grew under anaerobic and microaerophilic conditions but grew extremely poorly under aerobic conditions. The ether lipids were diphytanyl derivatives. The DNA G+C content of the type strain was 61.7 mol%. On the basis of the phylogenetic data and physiological and biochemical characteristics, strain SARL4B(T) represents a novel species of the genus Halorhabdus, for which the name Halorhabdus tiamatea is proposed. The type strain is SARL4B(T) (=DSM 18392(T)=JCM 14471(T)). An emended description of the genus Halorhabdus is also proposed.
从红海北部沙班海渊卤水-沉积物界面的一个样本中分离出了一种极端嗜盐古菌。对其16S rRNA基因序列进行系统发育分析表明，它与犹他嗜盐杆菌（Halorhabdus utahensis）（相似度为99.3%）关系密切，犹他嗜盐杆菌是嗜盐杆菌属（Halorhabdus）的唯一物种。菌株SARL4B(T)形成无色菌落，在45℃、27%（w/v）NaCl以及pH 6.5 - 7.0条件下生长最佳。该微生物利用一些复杂底物（如酵母提取物和淀粉）进行生长。菌株SARL4B(T)在厌氧和微需氧条件下生长，但在好氧条件下生长极差。醚脂为二植烷衍生物。模式菌株的DNA G+C含量为61.7 mol%。基于系统发育数据以及生理生化特征，菌株SARL4B(T)代表嗜盐杆菌属的一个新物种，为此提出将其命名为蒂阿马特嗜盐杆菌（Halorhabdus tiamatea）。模式菌株为SARL4B(T)（=DSM 18392(T)=JCM 14471(T)）。同时还提出了嗜盐杆菌属的修订描述。
