Le Doare Kirsty, Taylor Stephen, Allen Lauren, Gorringe Andrew, Heath Paul T, Kampmann Beate, Hesseling Anneke C, Jones Christine E
aCentre for International Child Health and Department of Academic Paediatrics, Imperial College, London bPaediatric Infectious Diseases Research Group, Institute for Infection and Immunity St George's, University of London, London, UK cVaccines and Immunity Theme, Medical Research Council Unit, Fajara, The Gambia dPublic Health England, Porton Down, Salisbury, UK eDepartment of Pediatrics and Child Health, Desmond Tutu TB Center, Stellenbosch University fInstitute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
AIDS. 2016 Jan 28;30(3):471-5. doi: 10.1097/QAD.0000000000000923.
Placental antibody transfer is impaired in the context of HIV infection, which may render HIV-exposed, uninfected infants vulnerable to group B Streptococcus (GBS) disease. The GBS antibody response predominately consists of immunoglobulin G2 (IgG2) antibody. Thus we determined whether concentration and placental transfer of anti-GBS antibody subclasses was altered in HIV-infected compared with HIV-uninfected mothers.
A retrospective analysis of anti-GBS antibody subclasses in 38 HIV-infected and 33 HIV-uninfected mothers and their uninfected infants.
Sera were analysed using a novel flow cytometric assay that quantified binding of IgG1, IgG2, IgG3 and IgG4 to serotype (ST)Ia, STIII and STV GBS bacteria.
IgG2 binding to GBS STIa and V was lower in HIV-infected women compared with HIV-uninfected women. Moreover, IgG2 binding to GBS STIa was also lower in HIV-exposed, uninfected infants compared with unexposed infants. However, there were no statistically significant differences in the transplacental transfer ratio of IgG2 for any GBS serotype. The transplacental transfer of total IgG was reduced for GBS STIII and V and IgG1 subclass for STIII; placental transfer of all other subclasses was comparable in HIV-affected and HIV-unaffected pregnancies.
Anti-GBS IgG2 placental transfer is not affected by HIV infection. This is important for functional antibody against the capsular polysaccharide of GBS and provides confidence that maternal GBS vaccination may result in functional activity in HIV-infected and uninfected women.
在HIV感染的情况下，胎盘抗体转移会受到损害，这可能使暴露于HIV但未感染的婴儿易患B族链球菌（GBS）疾病。GBS抗体反应主要由免疫球蛋白G2（IgG2）抗体组成。因此，我们确定与未感染HIV的母亲相比，感染HIV的母亲中抗GBS抗体亚类的浓度和胎盘转移是否发生了改变。
对38名感染HIV的母亲、33名未感染HIV的母亲及其未感染的婴儿的抗GBS抗体亚类进行回顾性分析。
使用一种新型流式细胞术检测法分析血清，该方法可量化IgG1、IgG2、IgG3和IgG4与血清型（ST）Ia、STIII和STV GBS细菌的结合情况。
与未感染HIV的女性相比，感染HIV的女性中IgG2与GBS STIa和V的结合较低。此外，与未暴露的婴儿相比，暴露于HIV但未感染的婴儿中IgG2与GBS STIa的结合也较低。然而，对于任何GBS血清型，IgG2的胎盘转移率没有统计学上的显著差异。GBS STIII和V的总IgG胎盘转移减少，STIII的IgG1亚类胎盘转移减少；在受HIV影响和未受HIV影响的妊娠中，所有其他亚类的胎盘转移相当。
抗GBS IgG2胎盘转移不受HIV感染的影响。这对于针对GBS荚膜多糖的功能性抗体很重要，并使人相信母体GBS疫苗接种可能会在感染HIV和未感染HIV的女性中产生功能活性。
