Neudecker T, Eder E, Deininger C, Henschler D
Institute of Toxicology, University of Würzburg, Federal Republic of Germany.
Environ Mol Mutagen. 1989;14(3):146-8. doi: 10.1002/em.2850140303.
The mutagenicity of crotonaldehyde in Salmonella typhimurium TA100 cannot be demonstrated in the standard plate incorporation assay. However, as reported earlier by our group, this alpha, beta-unsaturated aldehyde is clearly mutagenic in the liquid assay modification of this testing procedure. Carried out because of the doubts recently expressed by Cooper et al. (Environ Mutagen 9:289-295, 1987) concerning the observed mutagenicity of crotonaldehyde in S. typhimurium TA100, this study confirms that this compound is clearly a direct (without activation by mammalian microsomes)-acting mutagen in S. typhimurium TA100 under appropriate conditions in the preincubation assay. The observed mutagenicity is increased by extended preincubation time and increased bacterial cell densities.
在鼠伤寒沙门氏菌TA100中，巴豆醛的致突变性在标准平板掺入试验中无法得到证实。然而，正如我们小组之前所报道的，在该检测程序的液体试验改进方法中，这种α,β-不饱和醛具有明显的致突变性。由于库珀等人（《环境诱变剂》9:289 - 295, 1987）最近对在鼠伤寒沙门氏菌TA100中观察到的巴豆醛致突变性表示怀疑，开展了本研究，该研究证实，在预孵育试验的适当条件下，该化合物在鼠伤寒沙门氏菌TA100中显然是一种直接（无需哺乳动物微粒体激活）作用的诱变剂。延长预孵育时间和增加细菌细胞密度会增加观察到的致突变性。
