Liu Zhouying, Huang Jian, Huo Youping, Gong Jing, Zhang Yinhui, Wei Cong, Pu Jielin
State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei-Li-Shi Road, Xi-Cheng, Beijing 100037, China.
Integration of Traditional and Western Medical Research Academy of Hebei Province, Shijiazhuang 050035, China.
Evid Based Complement Alternat Med. 2015;2015:385953. doi: 10.1155/2015/385953. Epub 2015 Dec 3.
The present study tries to identify proteins implicated in bradycardia rabbits in hearts after ShenSongYangXin (SSYX, a traditional Chinese medicine) treatment. Eighteen adult rabbits were randomly assigned to three groups: sham, model, and SSYX treatment groups. Heart rate was recorded in rabbits and proteins were isolated from ventricular muscle. We used isobaric tags for elative and absolute quantitation (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry to identify altered proteins after SSYX treatment. The heart rate decreased after six weeks due to the injury of the sinoatrial node in the model group. This effect was partially reversed by 4-week SSYX treatment. A total of a2988 proteins were quantified by performing the iTRAQ-based experiments. Of these, 86 proteins were differentially expressed according to our criteria (42 upregulated and 44 downregulated). The identification of key proteins implicated in the treatment of bradycardia could serve as a foundation to better understand and further explore the molecular mechanism of SSYX treatment.
本研究试图鉴定参松养心（SSYX，一种中药）治疗后家兔心脏中与心动过缓相关的蛋白质。18只成年家兔被随机分为三组：假手术组、模型组和SSYX治疗组。记录家兔心率，并从心室肌中分离蛋白质。我们使用同重标签相对与绝对定量（iTRAQ）结合二维液相色谱-串联质谱法来鉴定SSYX治疗后发生变化的蛋白质。由于模型组窦房结损伤，六周后心率下降。4周的SSYX治疗部分逆转了这种效应。通过基于iTRAQ的实验共定量了2988种蛋白质。其中，根据我们的标准，有86种蛋白质差异表达（42种上调，44种下调）。鉴定与心动过缓治疗相关的关键蛋白质可为更好地理解和进一步探索SSYX治疗的分子机制奠定基础。
