Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.
Nat Chem Biol. 2016 Mar;12(3):138-40. doi: 10.1038/nchembio.2002. Epub 2016 Jan 18.
Engineered orthogonal translation systems have greatly enabled the expansion of the genetic code using noncanonical amino acids (NCAAs). However, the impact of NCAAs on organismal evolution remains unclear, in part because it is difficult to force the adoption of new genetic codes in organisms. By reengineering TEM-1 β-lactamase to be dependent on a NCAA, we maintained bacterial NCAA dependence for hundreds of generations without escape.
工程正交翻译系统极大地扩展了非天然氨基酸（NCAAs）在遗传密码子中的应用。然而，NCAAs 对生物进化的影响仍不清楚，部分原因是难以迫使生物体采用新的遗传密码子。通过对 TEM-1 β-内酰胺酶进行重新设计使其依赖于 NCAA，我们在没有逃逸的情况下使细菌 NCAA 依赖性维持了数百代。
