Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Br J Dermatol. 2016 Jun;174(6):1299-307. doi: 10.1111/bjd.14409. Epub 2016 Apr 20.
Acne vulgaris is a multifactorial disorder of the pilosebaceous units. Several studies have reported that insulin-like growth factor (IGF)-1, forkhead box transcription factor (Fox)O1 and mammalian target of rapamycin (mTOR) interactions may be the key to understanding the links between genetic and environmental factors in acne vulgaris.
To evaluate the immunohistochemical detection of mTOR and FoxO1 in the skin, and the serum level of IGF-1 in patients with acne vulgaris.
This study was carried out on 60 participants, including 40 patients with acne and 20 controls. A diet questionnaire was administered to the patients and controls. Serum levels of IGF-1 were measured using enzyme-linked immunosorbent assay, and skin biopsies were taken from lesions on the backs of the patients and controls. FoxO1 and mTOR expression was detected using immunohistochemistry.
A significantly higher serum IGF-1 level was found in the patients with acne than in the controls. The cytoplasmic expression of FoxO1 was found to be significantly greater in the acne group, whereas in the control subjects this expression was likely to be nuclear. Both the cytoplasmic expression and the nuclear expression of mTOR were significantly more intense in the patients with acne than in the controls. Excess consumption of a high-glycaemic-load diet was significantly associated with higher serum levels of IGF-1 and cytoplasmic expression of FoxO1 and mTOR.
These results suggest that FoxO1, mTOR, serum IGF-1 and a high-glycaemic-load diet may play a role in acne pathogenesis.
寻常痤疮是一种多因素的毛囊皮脂腺单位疾病。有几项研究报告称，胰岛素样生长因子 (IGF)-1、叉头框转录因子 (Fox)O1 和哺乳动物雷帕霉素靶蛋白 (mTOR) 的相互作用可能是理解寻常痤疮中遗传和环境因素之间联系的关键。
评估 mTOR 和 FoxO1 在皮肤中的免疫组织化学检测以及寻常痤疮患者血清 IGF-1 水平。
本研究共纳入 60 名参与者，包括 40 名痤疮患者和 20 名对照者。对患者和对照者进行饮食问卷。采用酶联免疫吸附试验测量血清 IGF-1 水平，并从患者和对照者背部皮损处取皮肤活检。采用免疫组织化学检测 FoxO1 和 mTOR 的表达。
痤疮患者的血清 IGF-1 水平明显高于对照者。在痤疮组中发现 FoxO1 的细胞质表达明显更高，而在对照组中 FoxO1 的表达可能是核内的。痤疮患者的 mTOR 细胞质表达和核内表达均明显强于对照组。高血糖负荷饮食的过量摄入与血清 IGF-1 水平升高以及 FoxO1 和 mTOR 的细胞质表达增加显著相关。
这些结果表明，FoxO1、mTOR、血清 IGF-1 和高血糖负荷饮食可能在痤疮发病机制中起作用。
