Xin Weijuan, Liu Xiaoxia, Ding Jingxin, Zhao Jing, Zhou Yang, Wu Qianyu, Hua Keqin
Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital of Fudan University Shanghai 200032, China.
Department of Regenerative Medicine, Tongji University School of Medicine Shanghai 200092, China.
Am J Transl Res. 2015 Nov 15;7(11):2433-41. eCollection 2015.
This study was to investigate the roles of lncRNA associated competitive endogenous RNAs (ceRNAs) network in the endometrial cancer (EC).
StarbaseV2.0 online software was used to predict the most probable lncRNAs which contain miR-205-5p binding site and are competent to interact with miR-205-5p. Then, lncRNAs which had decreased expression in EC compared with normal endometrium and conformed to the polyadenylated characteristics of lncRNAs were selected and then lncRNAs associated with miR-205-5p-PTEN network were identified.
Two novel genes RP11-395G23.3 and LA16c-313D11.11 associated with endometrial cancer were identified and proved to be non-coding RNAs. They were more effective ceRNAs associated with the miR-205-5p-PTEN network.
Our results suggest that lncRNAs harbor MREs (miRNA response elements) and play important roles in the post-transcriptional regulation in EC.
本研究旨在探讨长链非编码RNA相关的竞争性内源性RNA(ceRNA)网络在子宫内膜癌(EC)中的作用。
使用StarbaseV2.0在线软件预测最有可能含有miR-205-5p结合位点并能与miR-205-5p相互作用的长链非编码RNA。然后,选择与正常子宫内膜相比在EC中表达降低且符合长链非编码RNA多聚腺苷酸化特征的长链非编码RNA,进而鉴定与miR-205-5p-PTEN网络相关的长链非编码RNA。
鉴定出两个与子宫内膜癌相关的新基因RP11-395G23.3和LA16c-313D11.11,并证明它们是非编码RNA。它们是与miR-205-5p-PTEN网络相关的更有效的ceRNA。
我们的结果表明,长链非编码RNA含有微小RNA反应元件(MRE),并在子宫内膜癌的转录后调控中发挥重要作用。