Zhang Bin, Cai Yunlang, Shang Luoran, Wang Huan, Cheng Yao, Rong Fei, Gu Zhongze, Zhao Yuanjin
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Nanoscale. 2016 Feb 14;8(6):3841-7. doi: 10.1039/c5nr06368j. Epub 2016 Jan 27.
Diagnosing hematological disorders based on the separation and detection of cells in the patient's blood is a significant challenge. We have developed a novel barcode particle-based suspension array that can simultaneously capture and detect multiple types of blood cells. The barcode particles are polyacrylamide (PAAm) hydrogel inverse opal microcarriers with characteristic reflection peak codes that remain stable during cell capture on their surfaces. The hydrophilic PAAm hydrogel scaffolds of the barcode particles can entrap various plasma proteins to capture different cells in the blood, with little damage to captured cells.
基于患者血液中细胞的分离和检测来诊断血液系统疾病是一项重大挑战。我们开发了一种新型的基于条形码颗粒的悬浮阵列，它可以同时捕获和检测多种类型的血细胞。条形码颗粒是具有特征反射峰编码的聚丙烯酰胺（PAAm）水凝胶反蛋白石微载体，在其表面捕获细胞的过程中这些编码保持稳定。条形码颗粒的亲水性PAAm水凝胶支架可以截留各种血浆蛋白以捕获血液中的不同细胞，对捕获的细胞几乎没有损伤。
