孟加拉国伯克霍尔德菌临床分离株的抗菌药敏模式
Antimicrobial susceptibility pattern of clinical isolates of Burkholderia pseudomallei in Bangladesh.
作者信息
Dutta Subarna, Haq Sabah, Hasan Mohammad Rokibul, Haq Jalaluddin Ashraful
机构信息
Department of Microbiology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, 122 Kazi Nazrul Islam Avenue, Dhaka, 1000, Bangladesh.
Department of Microbiology, Ibrahim Medical College, 122 Kazi Nazrul Islam Avenue, Dhaka, 1000, Bangladesh.
出版信息
BMC Res Notes. 2017 Jul 20;10(1):299. doi: 10.1186/s13104-017-2626-5.
BACKGROUND
Melioidosis an infectious disease, caused by a Gram negative bacterium called Burkholderia pseudomallei, is endemic in Bangladesh. This organism is sensitive to limited number of antimicrobial agents and need prolonged treatment. There is no comprehensive data on the antimicrobial susceptibility profile of B. pseudomallei isolated in Bangladesh over last several years. The present study aimed to determine the antimicrobial susceptibility pattern of B. pseudomallei isolated in a tertiary care hospital of Dhaka city from 2009 to 2015.
METHODS
All B. pseudomallei isolated from melioidosis patients over a period of 7 years (2009-2015) in the Department of Microbiology of a 725-bed tertiary care referral hospital in Dhaka city, Bangladesh were included in the study. B. pseudomallei was identified by Gram stain, culture, specific biochemical tests, serology and PCR using specific primers constructed from 16s rRNA region of B. pseudomallei. Antimicrobial susceptibility to specific agents was determined by disk diffusion and minimum inhibitory concentration methods.
RESULTS
A total of 20 isolates of B. pseudomallei which were isolated from patients coming from different geographic locations of Bangladesh were included in the study. All the isolates were uniformly sensitive (100%) to ceftazidime, imipenem, piperacillin-tazobactam, amoxicillin-clavulanic acid and tetracycline by both disk diffusion and MIC methods. Two strains were resistant to trimethoprim-sulfamethoxazole by disk diffusion method but were sensitive by MIC method. The MIC and MIC values of the above antimicrobial agents were almost similar. All the isolates were resistant to amikacin by both MIC and disk diffusion methods.
CONCLUSION
The results of the study suggest that B. pseudomallei prevalent in Bangladesh were still susceptible to all recommended antimicrobial agents used for the treatment of melioidosis. However, regular monitoring is needed to detect any emergence of resistance and shifting of MIC and MIC values.
背景
类鼻疽是一种由革兰氏阴性菌伯克霍尔德菌引起的传染病，在孟加拉国呈地方性流行。这种病原体对有限数量的抗菌药物敏感，且需要长期治疗。过去几年，孟加拉国分离出的伯克霍尔德菌的抗菌药物敏感性概况尚无全面数据。本研究旨在确定2009年至2015年在达卡市一家三级护理医院分离出的伯克霍尔德菌的抗菌药物敏感性模式。
方法
本研究纳入了孟加拉国达卡市一家拥有725张床位的三级护理转诊医院微生物科在7年（2009 - 2015年）期间从类鼻疽患者中分离出的所有伯克霍尔德菌。通过革兰氏染色、培养、特定生化试验、血清学以及使用从伯克霍尔德菌16s rRNA区域构建的特异性引物进行PCR来鉴定伯克霍尔德菌。通过纸片扩散法和最低抑菌浓度法测定对特定药物的抗菌药物敏感性。
结果
本研究纳入了从孟加拉国不同地理位置的患者中分离出的总共20株伯克霍尔德菌。通过纸片扩散法和MIC法，所有分离株对头孢他啶、亚胺培南、哌拉西林 - 他唑巴坦、阿莫西林 - 克拉维酸和四环素均呈一致敏感（100%）。通过纸片扩散法，有两株菌株对甲氧苄啶 - 磺胺甲恶唑耐药，但通过MIC法敏感。上述抗菌药物的MIC和MIC值几乎相似。通过MIC法和纸片扩散法，所有分离株对阿米卡星均耐药。
结论
研究结果表明，孟加拉国流行的伯克霍尔德菌对所有推荐用于治疗类鼻疽的抗菌药物仍敏感。然而，需要定期监测以检测耐药性的任何出现以及MIC和MIC值的变化。
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