Taylor Bryan F, Ramirez Harvey E, Battles August H, Andrutis Karl A, Neubert John K
Animal Care Services, University of Florida, Gainesville, Florida.
Animal Care Services, University of Florida, Gainesville, Florida;, Email:
J Am Assoc Lab Anim Sci. 2016 Jan;55(1):74-82.
Effective pain management for rats and mice is crucial due to the continuing increase in the use of these species in biomedical research. Here we used a recently validated operant orofacial pain assay to determine dose-response curves for buprenorphine and tramadol when mixed in nut paste and administered to male and female rats. Statistically significant analgesic doses of tramadol in nut paste included doses of 20, 30, and 40 mg/kg for female rats but only 40 mg/kg for male rats. For male rats receiving buprenorphine mixed in nut paste, a significant analgesic response was observed at 0.5 and 0.6 mg/kg. None of the doses tested produced a significant analgesic response in female rats. Our results indicate that at the doses tested, tramadol and buprenorphine produced an analgesic response in male rats. In female rats, tramadol shows a higher analgesic effect than buprenorphine. The analgesic effects observed 60 min after administration of the statistically significant oral doses of both drugs were similar to the analgesic effects of 0.03 mg/kg subcutaneous buprenorphine 30 min after administration. The method of voluntary ingestion could be effective, is easy to use, and would minimize stress to the rats during the immediate postoperative period.
由于在生物医学研究中使用大鼠和小鼠的情况持续增加，对它们进行有效的疼痛管理至关重要。在这里，我们使用了一种最近验证的操作性口腔面部疼痛测定法，以确定丁丙诺啡和曲马多与坚果酱混合并给予雄性和雌性大鼠时的剂量反应曲线。坚果酱中曲马多的统计学显著镇痛剂量包括雌性大鼠的20、30和40mg/kg，但雄性大鼠仅为40mg/kg。对于接受与坚果酱混合的丁丙诺啡的雄性大鼠，在0.5和0.6mg/kg时观察到显著的镇痛反应。测试的所有剂量在雌性大鼠中均未产生显著的镇痛反应。我们的结果表明，在所测试的剂量下，曲马多和丁丙诺啡在雄性大鼠中产生了镇痛反应。在雌性大鼠中，曲马多的镇痛效果比丁丙诺啡更高。在给予两种药物的统计学显著口服剂量60分钟后观察到的镇痛效果与皮下注射0.03mg/kg丁丙诺啡30分钟后的镇痛效果相似。自愿摄入的方法可能是有效的，易于使用，并且会在术后即刻将对大鼠的应激降至最低。
