Luan Yunxia, Chen Jiayi, Li Cheng, Xie Gang, Fu Hailong, Ma Zhihong, Lu Anxiang
Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China.
Toxins (Basel). 2015 Dec 10;7(12):5377-85. doi: 10.3390/toxins7124883.
A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.
利用阳离子聚合物和金纳米颗粒（AuNPs）开发了一种基于无标记适体的方法，用于高灵敏度和特异性检测赭曲霉毒素A（OTA）。OTA适体用作识别元件，基于阳离子聚合物使AuNPs聚集，进行OTA的比色检测。通过光谱定量分析，该比色测定法在存在其他干扰毒素的情况下，能够以高选择性检测低至0.009 ng/mL的OTA。本研究为OTA检测提供了一种快速且灵敏的视觉检测方法的新选择。
