Cheng Tao, Zhang Jian-Guo, Cheng Yuan-He, Gao Zhong-Wei, Ren Xiao-Qiang
Department of Urology, the 1st Affiliated Hospital of He'nan University of Science and Technology, Luoyang, China.
Asian Pac J Cancer Prev. 2012;13(6):2681-5. doi: 10.7314/apjcp.2012.13.6.2681.
This study focused on PTEN and Livin expression and associations with malignancy in human renal clear cell carcinomas (RCCC).
PTEN and Livin expression was assessed in 100 RCCC tissue samples, 50 paracarcinoma cases, and 20 normal renal tissue samples using the immunohistochemical Streptavidin proxidase (SP) method. The relationships between binding and corresponding biological characteristics, such as histological grade, lymph node metastases, and clinical stages were analyzed.
Positive PTEN expression in RCCC was significantly lower than in renal tissue adjacent to carcinoma tissue and normal renal tissue (P<0.01). Livin expression in the renal tissue adjacent to the carcinoma and normal renal tissues exhibited only low levels, whereas overall Livin expression in RCCC was statistically significant (P<0.01). In RCCC, PTEN expression rate gradually decreased with an increase in clinical stage, whereas that of Livin increased to statistically significant levels (P<0.01), PTEN and Livin levels being negatively correlated (r=-0.395, P<0.01).
PTEN and Livin are important in RCCC development. The two factors combined are expected to provide indices for estimating RCCC malignancy and progression levels, as well as references for RCCC diagnosis and treatment.
本研究聚焦于人类肾透明细胞癌（RCCC）中PTEN和Livin的表达及其与恶性肿瘤的关系。
采用免疫组织化学链霉亲和素过氧化物酶（SP）法，对100例RCCC组织样本、50例癌旁组织样本和20例正常肾组织样本中的PTEN和Livin表达进行评估。分析其与组织学分级、淋巴结转移及临床分期等相应生物学特征之间的关系。
RCCC中PTEN阳性表达显著低于癌旁肾组织和正常肾组织（P<0.01）。癌旁肾组织和正常肾组织中Livin仅呈低水平表达，而RCCC中Livin总体表达具有统计学意义（P<0.01）。在RCCC中，PTEN表达率随临床分期增加而逐渐降低，而Livin表达率则升高至具有统计学意义的水平（P<0.01），PTEN与Livin水平呈负相关（r=-0.395，P<0.01）。
PTEN和Livin在RCCC发生发展中具有重要作用。这两个因素有望为评估RCCC的恶性程度和进展水平提供指标，也可为RCCC的诊断和治疗提供参考。
