Ashraf Jalaluddin M, Ansari Mohammad Azam, Khan Haris M, Alzohairy Mohammad A, Choi Inho
School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
Nanotechnology and Antimicrobial Drug Resistance Research Laboratory, Department of Microbiology, Jawaharlal Nehru Medical College &Hospital, Aligarh Muslim University, Aligarh-202002, U.P., India.
Sci Rep. 2016 Feb 2;6:20414. doi: 10.1038/srep20414.
Advanced glycation end-products (AGEs) resulting from non-enzymatic glycation are one of the major factors implicated in secondary complications of diabetes. Scientists are focusing on discovering new compounds that may be used as potential AGEs inhibitors without affecting the normal structure and function of biomolecules. A number of natural and synthetic compounds have been proposed as AGE inhibitors. In this study, we investigated the inhibitory effects of AgNPs (silver nanoparticles) in AGEs formation. AgNPs (~30.5 nm) synthesized from Aloe Vera leaf extract were characterized using UV-Vis spectroscopy, energy-dispersive X-ray spectroscopy (EDX), high resolution-transmission electron microscopy, X-ray diffraction and dynamic light scattering (DLS) techniques. The inhibitory effects of AgNPs on AGEs formation were evaluated by investigating the degree of reactivity of free amino groups (lysine and arginine residues), protein-bound carbonyl and carboxymethyl lysine (CML) content, and the effects on protein structure using various physicochemical techniques. The results showed that AgNPs significantly inhibit AGEs formation in a concentration dependent manner and that AgNPs have a positive effect on protein structure. These findings strongly suggest that AgNPs may play a therapeutic role in diabetes-related complications.
由非酶糖基化产生的晚期糖基化终产物（AGEs）是糖尿病继发性并发症的主要因素之一。科学家们正致力于发现可作为潜在AGEs抑制剂的新化合物，同时不影响生物分子的正常结构和功能。已有多种天然和合成化合物被提议作为AGE抑制剂。在本研究中，我们研究了银纳米颗粒（AgNPs）对AGEs形成的抑制作用。使用紫外可见光谱、能量色散X射线光谱（EDX）、高分辨率透射电子显微镜、X射线衍射和动态光散射（DLS）技术对从芦荟叶提取物合成的AgNPs（约30.5纳米）进行了表征。通过研究游离氨基（赖氨酸和精氨酸残基）的反应程度、蛋白质结合羰基和羧甲基赖氨酸（CML）含量以及使用各种物理化学技术对蛋白质结构的影响，评估了AgNPs对AGEs形成的抑制作用。结果表明，AgNPs以浓度依赖的方式显著抑制AGEs的形成，并且AgNPs对蛋白质结构有积极影响。这些发现强烈表明，AgNPs可能在糖尿病相关并发症中发挥治疗作用。
