Price Aryn A, Grakoui Arash, Weiss David S
Department of Microbiology and Immunology, Microbiology and Molecular Genetics Program, Emory University, Atlanta, GA 30329, USA; Emory Vaccine Center, Emory University, Atlanta, GA 30329, USA; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.
Emory Vaccine Center, Emory University, Atlanta, GA 30329, USA; Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30329, USA.
Trends Microbiol. 2016 Apr;24(4):294-306. doi: 10.1016/j.tim.2016.01.005. Epub 2016 Feb 3.
Clustered, regularly interspaced, short palindromic repeats - CRISPR-associated (CRISPR-Cas) systems - are sequence-specific RNA-directed endonuclease complexes that bind and cleave nucleic acids. These systems evolved within prokaryotes as adaptive immune defenses to target and degrade nucleic acids derived from bacteriophages and other foreign genetic elements. The antiviral function of these systems has now been exploited to combat eukaryotic viruses throughout the viral life cycle. Here we discuss current advances in CRISPR-Cas9 technology as a eukaryotic antiviral defense.
成簇规律间隔短回文重复序列 - 与CRISPR相关的(CRISPR-Cas)系统 - 是结合并切割核酸的序列特异性RNA导向的核酸内切酶复合物。这些系统在原核生物中进化,作为适应性免疫防御机制,以靶向和降解来自噬菌体和其他外来遗传元件的核酸。现在,这些系统的抗病毒功能已被用于在整个病毒生命周期中对抗真核病毒。在此,我们讨论CRISPR-Cas9技术作为真核抗病毒防御的当前进展。