Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, Guangdong, China.
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Agriculture, South China Agricultural University, Guangzhou, Guangdong, China.
Plant Biotechnol J. 2018 Aug;16(8):1415-1423. doi: 10.1111/pbi.12881. Epub 2018 Feb 14.
Recently, CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system has been used to produce plants resistant to DNA virus infections. However, there is no RNA virus control method in plants that uses CRISPR-Cas system to target the viral genome directly. Here, we reprogrammed the CRISPR-Cas9 system from Francisella novicida to confer molecular immunity against RNA viruses in Nicotiana benthamiana and Arabidopsis plants. Plants expressing FnCas9 and sgRNA specific for the cucumber mosaic virus (CMV) or tobacco mosaic virus (TMV) exhibited significantly attenuated virus infection symptoms and reduced viral RNA accumulation. Furthermore, in the transgenic virus-targeting plants, the resistance was inheritable and the progenies showed significantly less virus accumulation. These data reveal that the CRISPR/Cas9 system can be used to produce plant that stable resistant to RNA viruses, thereby broadening the use of such technology for virus control in agricultural field.
最近，CRISPR-Cas（成簇、规律间隔短回文重复序列-CRISPR 相关蛋白）系统已被用于生产抗 DNA 病毒感染的植物。然而，目前还没有利用 CRISPR-Cas 系统直接靶向病毒基因组来控制植物中 RNA 病毒的方法。在这里，我们重新编程了弗朗西斯氏菌 novicida 的 CRISPR-Cas9 系统，使其在黄花烟和拟南芥植物中获得针对 RNA 病毒的分子免疫力。表达 FnCas9 和针对黄瓜花叶病毒（CMV）或烟草花叶病毒（TMV）的 sgRNA 的植物表现出明显减轻的病毒感染症状和减少的病毒 RNA 积累。此外，在转基因病毒靶向植物中，抗性是可遗传的，后代的病毒积累明显减少。这些数据表明，CRISPR/Cas9 系统可用于生产稳定抗 RNA 病毒的植物，从而拓宽了该技术在农业领域控制病毒的应用。
