Uchiyama R C, Osborn T G, Moore T L
Department of Internal Medicine, St. Louis University School of Medicine, MO 63104.
J Rheumatol. 1989 Aug;16(8):1074-8.
Sera from 12 patients with juvenile rheumatoid arthritis (JRA) with active iridocyclitis were incubated with frozen, sectioned, whole human eye tissue. Antibodies were detected using fluorescein conjugated goat F(ab')2 antibody to human IgG by immunofluorescent microscopy. Immunofluorescence was determined on tissue from the iris, retina, and 3 portions of the ciliary body. Sera of patients with JRA with iridocyclitis were compared to sera from patients with JRA with and without antinuclear antibodies (ANA) and healthy children. An increased frequency of antibody to the human iris was seen with sera of patients with JRA with iridocyclitis compared to healthy children's sera. A higher frequency of antibody was also noted to human retina in sera of patients with JRA with iridocyclitis compared to patients without ANA and healthy children. No increased frequency of antibody was detected to ciliary body. Sera of 7 patients with JRA with iridocyclitis were also compared during a time of inactive eye disease to a time of active disease. No difference in binding to eye tissue was detected at times of inactive disease compared to controls. Our results demonstrate the presence of antibody to iris and retina by immunofluorescence in the sera of patients with JRA with iridocyclitis.
将12例患有活动性虹膜睫状体炎的青少年类风湿性关节炎（JRA）患者的血清与冷冻切片的完整人眼组织一起孵育。通过免疫荧光显微镜，使用荧光素偶联的山羊抗人IgG F(ab')2抗体检测抗体。在虹膜、视网膜和睫状体的3个部分的组织上测定免疫荧光。将患有虹膜睫状体炎的JRA患者的血清与患有和不患有抗核抗体（ANA）的JRA患者以及健康儿童的血清进行比较。与健康儿童的血清相比，患有虹膜睫状体炎的JRA患者的血清中抗人虹膜抗体的频率增加。与无ANA的患者和健康儿童相比，患有虹膜睫状体炎的JRA患者的血清中抗人视网膜抗体的频率也更高。未检测到抗睫状体抗体频率增加。还比较了7例患有虹膜睫状体炎的JRA患者在眼部疾病不活动期和活动期的血清。与对照组相比，在疾病不活动期未检测到与眼组织结合的差异。我们的结果表明，通过免疫荧光法在患有虹膜睫状体炎的JRA患者血清中检测到抗虹膜和抗视网膜抗体。
