Pauls J D, Silverman E, Laxer R M, Fritzler M J
Joint Injury and Diseases Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada.
Arthritis Rheum. 1989 Jul;32(7):877-83.
The specificity of juvenile rheumatoid arthritis (JRA) sera for histone subclasses was examined by immunoblotting. Antibodies to H1 alone were found in 4 of 21 pauciarticular-onset JRA sera, 4 of 19 polyarticular-onset JRA sera, and 2 of 11 systemic-onset JRA sera. Antibodies to H5 alone were found in 1 of 21 pauciarticular JRA sera, 1 of 19 polyarticular JRA sera, and 3 of 11 systemic JRA sera. Antibodies to both H1 and H5 were found in 4 of 21 pauciarticular JRA sera, 4 of 19 polyarticular JRA sera, and 1 of 11 systemic JRA sera. Antibodies to the core histones (H2A and H2B) were found in 1 of 21 pauciarticular JRA sera, 1 of 19 polyarticular JRA sera, and no systemic JRA sera. No reactivity to histones was observed in 30 sera from age-matched children with nonrheumatic diseases. The presence of H1 and H5 antibodies did not correlate with antinuclear antibody titers or with a homogeneous pattern of immunofluorescence. The predominance of H1 and H5 antibodies and relative absence of antibodies binding to core histones in JRA contrast with findings in adult systemic lupus erythematosus. Further, the presence of antibodies to H5 alone in some of the JRA patients indicates that the immune response in these patients is directed to determinants that are not shared by sequences of mammalian proteins.
通过免疫印迹法检测了青少年类风湿性关节炎（JRA）血清对组蛋白亚类的特异性。在21例少关节起病型JRA血清中有4例、19例多关节起病型JRA血清中有4例、11例全身起病型JRA血清中有2例发现了单独针对H1的抗体。在21例少关节JRA血清中有1例、19例多关节JRA血清中有1例、11例全身JRA血清中有3例发现了单独针对H5的抗体。在21例少关节JRA血清中有4例、19例多关节JRA血清中有4例、11例全身JRA血清中有1例发现了针对H1和H5两者的抗体。在21例少关节JRA血清中有1例、19例多关节JRA血清中有1例发现了针对核心组蛋白（H2A和H2B）的抗体，全身起病型JRA血清中未发现。在30例年龄匹配的非风湿性疾病儿童的血清中未观察到与组蛋白的反应性。H1和H5抗体的存在与抗核抗体滴度或免疫荧光的均质模式无关。JRA中H1和H5抗体占优势且与核心组蛋白结合的抗体相对缺乏，这与成人系统性红斑狼疮的发现形成对比。此外，一些JRA患者中单独存在针对H5的抗体表明这些患者的免疫反应针对的是哺乳动物蛋白质序列不共有的决定簇。
