Haber P L, Osborn T G, Moore T L
Department of Internal Medicine, Cardinal Glennon Children's Hospital, St. Louis, MO.
J Rheumatol. 1989 Jul;16(7):949-54.
To identify whether sera containing antinuclear antibody (ANA) from patients with juvenile rheumatoid arthritis (JRA) recognize a common antigen, extracts of HeLa nuclei were prepared by incubating the nuclei with buffers of sequentially increasing salt concentrations (0.1 M, 0.3 M, and 0.5 M NaCl). Western blot analysis using sera from 82 patients with JRA revealed that 63% of ANA positive JRA sera reacted with 2 bands (50-40 kDa) in the 0.3 M extract whereas only 14% of ANA negative JRA sera had antibody to these bands. Antibody to the 50-40 kDa antigen(s) strongly correlated with the presence of ANA by immunofluorescence and was found in 70% of patients with iritis; however, it did not correlate with disease subgroup or disease activity.
为了确定幼年类风湿性关节炎（JRA）患者中含有抗核抗体（ANA）的血清是否识别共同抗原，通过将细胞核与盐浓度依次增加（0.1M、0.3M和0.5M NaCl）的缓冲液孵育来制备HeLa细胞核提取物。使用82例JRA患者的血清进行蛋白质印迹分析显示，63%的ANA阳性JRA血清与0.3M提取物中的两条带（50 - 40kDa）反应，而只有14%的ANA阴性JRA血清具有针对这些条带的抗体。针对50 - 40kDa抗原的抗体通过免疫荧光与ANA的存在密切相关，并且在70%的虹膜炎患者中发现；然而，它与疾病亚组或疾病活动无关。
