Murray K J, Szer W, Grom A A, Donnelly P, Levinson J E, Giannini E H, Glass D N, Szer I S
Department of Pediatrics, Children's Hospital Medical Center, University of Cincinnati College of Medicine, OH 45229, USA.
J Rheumatol. 1997 Mar;24(3):560-7.
To study the frequency of autoantibodies to the 45 kDa DEK nuclear antigen, a putative oncoprotein, in a sample of patients with juvenile rheumatoid arthritis (JRA), and to make correlations with disease subtype and complications such as iridocyclitis. Class I and Class II HLA associations with reactivity to the antigen were also sought.
Sera from 146 HLA typed patients with JRA representing all subtypes were analyzed for reactivity with the 45 kDa DEK protein by immunoblotting. The antigen was purified to near homogeneity from nuclei of HeLa cells.
Antibodies to DEK were found in 57% of all patients with JRA compared to 3% of controls (p < 0.0001). Antibodies were detected more frequently in pauciarticular onset (78%) than in polyarticular onset patients (29%; p < 0.01) and controls (3%; p < 0.0001). 97% of patients with JRA (regardless of onset subtype) and iridocyclitis had anti-DEK antibodies compared to 47% of patients without eye disease (p < 0.0001). Anti-DEK antibodies were found more frequently in females compared to males in the pauciarticular onset disease group (84 vs 42%; p < 0.01). The occurrence of anti-DEK antibodies was closely associated with positive antinuclear antibody serology, and a strong association with the Class I gene HLA-A2 was also observed.
Antibodies to the 45 kDa DEK protein are characteristic of the pauciarticular onset subtype of JRA, particularly in patients with a history of iridocyclitis. The occurrence of anti-DEK antibodies is significantly but paradoxically associated with the presence of the HLA-A2 allele in such patients.
研究青少年类风湿性关节炎（JRA）患者样本中针对假定癌蛋白45 kDa DEK核抗原的自身抗体频率，并与疾病亚型以及虹膜炎等并发症进行相关性分析。同时也探寻I类和II类人类白细胞抗原（HLA）与该抗原反应性的关联。
通过免疫印迹法分析了146例代表所有亚型的HLA分型JRA患者血清与45 kDa DEK蛋白的反应性。该抗原从HeLa细胞核中纯化至接近均一性。
所有JRA患者中有57%检测到抗DEK抗体，而对照组为3%（p < 0.0001）。少关节起病患者中抗体检测频率更高（78%），高于多关节起病患者（29%；p < 0.01）和对照组（3%；p < 0.0001）。有虹膜炎的JRA患者（无论起病亚型）中97%有抗DEK抗体，而无眼部疾病的患者中这一比例为47%（p < 0.0001）。在少关节起病疾病组中，女性抗DEK抗体的检出频率高于男性（84%对42%；p < 0.01）。抗DEK抗体的出现与抗核抗体血清学阳性密切相关，并且还观察到与I类基因HLA - A2有强关联。
针对45 kDa DEK蛋白的抗体是JRA少关节起病亚型的特征，尤其是有虹膜炎病史的患者。在这类患者中，抗DEK抗体的出现与HLA - A2等位基因的存在显著但自相矛盾地相关。
