University of Ljubljana, Ljubljana 1000, Slovenia.
IEEE Trans Nanobioscience. 2011 Mar;10(1):36-43. doi: 10.1109/TNB.2011.2128340. Epub 2011 Apr 19.
We describe the development, fabrication and testing of a microfluidic chamber for dielectrophoretic field-flow separation of biological cells based on their electrical properties. The chamber was constructed from a single Pyrex wafer with interdigitated Au electrodes, a spacer, and a top cover glass, making the events in the chamber observable under most optical microscopes. The dimensions were optimized based on numerical computations of the electric field, its gradient and the fluid-flow velocity profile. The electrodes were fabricated using photolithography. A double-sided self-adhesive tape of 100 μm thickness was used as a spacer, with an opening of 80 mm length and 20 mm width cut in its middle to form a channel of 100 μm height, and with water-resistant acrylic glue of the tape holding the glass plates together and providing a tight seal. The glue loses its adhesive properties above 70 °C, allowing for easy disassembly of the chamber in hot water and its thorough cleaning. A 1:1 mixture of normal and 50 °C -heat-treated CHO cells was used to test the chamber. A 93% efficiency of separation was obtained, confirming the usefulness of the chamber in separating cells with sufficient differences in electrical properties of their membranes.
我们描述了一种基于细胞电学性质的介电泳场流分离生物细胞的微流控室的开发、制造和测试。该室由单个 Pyrex 晶片、叉指状 Au 电极、间隔物和上盖玻璃组成，使腔室内的事件在大多数光学显微镜下都可观察到。根据电场、其梯度和流体流动速度分布的数值计算对尺寸进行了优化。电极通过光刻制造。厚度为 100μm 的双面自粘胶带用作间隔物，在中间切开一个 80mm 长、20mm 宽的开口，形成一个 100μm 高的通道，并用胶带的耐水亚克力胶将玻璃板固定在一起并形成紧密的密封。当温度超过 70°C 时，胶水会失去粘性，从而可以在热水中轻松拆卸腔室并进行彻底清洁。使用正常和 50°C 热处理 CHO 细胞的 1:1 混合物来测试腔室。获得了 93%的分离效率，证实了该腔室在分离具有足够膜电性质差异的细胞方面的有用性。
