Zhao Yongfeng, Pang Bo, Luehmann Hannah, Detering Lisa, Yang Xuan, Sultan Deborah, Harpstrite Scott, Sharma Vijay, Cutler Cathy S, Xia Younan, Liu Yongjian
Department of Radiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30332, USA.
Adv Healthc Mater. 2016 Apr 20;5(8):928-35. doi: 10.1002/adhm.201500992. Epub 2016 Feb 10.
Gold nanoparticles have been labeled with various radionuclides and extensively explored for single photon emission computed tomography (SPECT) in the context of cancer diagnosis. The stability of most radiolabels, however, still needs to be improved for accurate detection of cancer biomarkers and thereby monitoring of tumor progression and metastasis. Here, the first synthesis of Au nanoparticles doped with (199)Au atoms for targeted SPECT tumor imaging in a mouse triple negative breast cancer (TNBC) model is reported. By directly incorporating (199)Au atoms into the crystal lattice of each Au nanoparticle, the stability of the radiolabel can be ensured. The synthetic procedure also allows for a precise control over both the radiochemistry and particle size. When conjugated with D-Ala1-peptide T-amide, the Au nanoparticles doped with (199)Au atoms can serve as a C-C chemokine receptor 5 (CCR5)-targeted nanoprobe for the sensitive and specific detection of both TNBC and its metastasis in a mouse tumor model.
金纳米颗粒已用各种放射性核素进行标记，并在癌症诊断的背景下广泛用于单光子发射计算机断层扫描（SPECT）。然而，为了准确检测癌症生物标志物并由此监测肿瘤进展和转移，大多数放射性标记的稳定性仍有待提高。在此，报道了首次合成掺杂有（199）Au原子的金纳米颗粒，用于在小鼠三阴性乳腺癌（TNBC）模型中进行靶向SPECT肿瘤成像。通过将（199）Au原子直接掺入每个金纳米颗粒的晶格中，可以确保放射性标记的稳定性。合成过程还允许对放射化学和颗粒大小进行精确控制。当与D-Ala1-肽T-酰胺缀合时，掺杂有（199）Au原子的金纳米颗粒可作为C-C趋化因子受体5（CCR5）靶向纳米探针，用于在小鼠肿瘤模型中灵敏且特异性地检测TNBC及其转移。
