Nayak Guruprasad, Honguntikar Sachin D, Kalthur Sneha Guruprasad, D'Souza Antony Sylvan, Mutalik Srinivas, Setty Manjunath M, Kalyankumar Raksha, Krishnamurthy Hanumanthappa, Kalthur Guruprasad, Adiga Satish Kumar
Center of Excellence in Clinical Embryology, Level 2, Central Research Lab, Kasturba Medical College, Manipal University, Manipal-576104, Karnataka, India.
Department of Anatomy, Kasturba Medical College, Manipal University, Manipal-576104, Karnataka, India.
J Ethnopharmacol. 2016 Apr 22;182:101-9. doi: 10.1016/j.jep.2016.02.003. Epub 2016 Feb 10.
Moringa oleifera Lam. is widely cultivated in Asian and African countries for its medicinal and dietary significance. The leaves are highly nutritious and are known to possess various biological activities.
Pre-pubertal Swiss albino male mice were injected with single dose of cyclophosphamide (CP, 200mg/kg body weight) or ethanolic extract of Moringa oleifera leaves (MOE, 100mg/kg body weight) intraperitoneally. In combination group, MOE was administered 24h prior to CP injection.
CP induced a significant decrease in testicular weight (p<0.01) and depletion of germ cells (p<0.001) and higher level of DNA damage (p<0.001) compared to control. The expression of P53, Bax, Cytochrome C (Cyt C) was increased while there was a decrease in the expression of Bcl2, c-Kit and Oct4. Administration of MOE 24h prior to CP treatment ameliorated the depletion (p<0.001), DNA damage (p<0.001) and apoptosis (p<0.01) of germ cells induced by CP. The mitigating effect of MOE appears to be mediated by up-regulating the expression of c-Kit and Oct4 transcripts in P53-independent manner.
MOE protects the spermatogonial cells from CP-induced damage by modulating the apoptotic response elicited by CP and therefore can be considered as an efficient method of male fertility preservation.
辣木在亚洲和非洲国家因其药用和饮食意义而被广泛种植。其叶子营养丰富，具有多种生物活性。
对青春期前的瑞士白化雄性小鼠腹腔注射单剂量的环磷酰胺（CP，200mg/kg体重）或辣木叶乙醇提取物（MOE，100mg/kg体重）。在联合组中，在注射CP前24小时给予MOE。
与对照组相比，CP导致睾丸重量显著降低（p<0.01）、生殖细胞耗竭（p<0.001）以及更高水平的DNA损伤（p<0.001）。P53、Bax、细胞色素C（Cyt C）的表达增加，而Bcl2、c-Kit和Oct4的表达降低。在CP治疗前24小时给予MOE可改善CP诱导的生殖细胞耗竭（p<0.001）、DNA损伤（p<0.001）和凋亡（p<0.01）。MOE的减轻作用似乎是以不依赖P53的方式上调c-Kit和Oct4转录本的表达来介导的。
MOE通过调节CP引发的凋亡反应来保护精原细胞免受CP诱导的损伤，因此可被视为一种有效的男性生育力保存方法。
