Zeng Bin, Luo Junyi, Wang Peng, Yang Lin, Chen Ting, Sun Jiajie, Xie Meiying, Li Meng, Zhang Haojie, He Jiajian, Zhang Yongliang, Xi Qianyun
Guangdong Provincial Key Laboratory of Animal Nutrition Control National Engineering Research Center For Breeding Swine Industry College of Animal Science South China Agricultural University Guangzhou China.
Guangdong Medical Laboratory Animal Center Foshan China.
Food Sci Nutr. 2019 Jan 22;7(2):738-746. doi: 10.1002/fsn3.918. eCollection 2019 Feb.
is a tropical plant with high nutritional and medicinal value. Recent studies have reported its remarkable effects in inflammatory, antioxidative, and anti-diabetes modulations, but there was no significant report on its role in animal breeding. In this study, we investigated the effects of dietary leaf (MOL) on reproductive performances in mice. We studied the reproductive performance of mice for six consecutive gestations. Mice fed with 4% MOL diet showed improved litter size, litter birth weight, and litter survivals until weaning age compared to control mice fed with normal diet (<0.05). Mice fed with MOL diet did not change weight and organ coefficients. Serum malondialdehyde (MDA) concentrations in both male and female mice were significantly decreased by dietary MOL (<0.05), but glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) were unchanged. For male, dietary MOL significantly reduced sperm abnormality rate (<0.05) and Bcl2-associated X protein (Bax) expression in testis (<0.05), but did not affect serum testosterone and the expression levels of androgen receptor (AR), phosphoglycerate kinase 2 (Pgk2), protamine2 (Prm2), and B cell leukemia/lymphoma 2 (Bcl2) in testis. For female, dietary MOL did not change serum estradiol and the expressions of estrogen receptor beta (ERβ), Bcl2, Bax, and vascular endothelial growth factor receptor (VEGFR) in ovary. In summary, MOL increased litter size and antioxidant ability, reduced the rate of sperm abnormality and the expression of Bax. Therefore, MOL may serve as a functional feed addictive for improving animal reproductive performance.
是一种具有高营养价值和药用价值的热带植物。最近的研究报道了其在炎症、抗氧化和抗糖尿病调节方面的显著作用，但关于其在动物繁殖中的作用尚无重要报道。在本研究中，我们研究了日粮中添加桑叶（MOL）对小鼠繁殖性能的影响。我们连续六个妊娠期研究了小鼠的繁殖性能。与喂食正常日粮的对照小鼠相比，喂食4% MOL日粮的小鼠窝产仔数、窝出生体重和断奶前窝仔存活率均有所提高（<0.05）。喂食MOL日粮的小鼠体重和器官系数没有变化。日粮中添加MOL可显著降低雄性和雌性小鼠血清丙二醛（MDA）浓度（<0.05），但谷胱甘肽过氧化物酶（GSH-PX）和超氧化物歧化酶（SOD）没有变化。对于雄性小鼠，日粮中添加MOL可显著降低精子畸形率（<0.05）和睾丸中Bcl2相关X蛋白（Bax）的表达（<0.05），但不影响血清睾酮以及睾丸中雄激素受体（AR）、磷酸甘油酸激酶2（Pgk2）、鱼精蛋白2（Prm2）和B细胞白血病/淋巴瘤2（Bcl2）的表达水平。对于雌性小鼠，日粮中添加MOL不改变血清雌二醇以及卵巢中雌激素受体β（ERβ）、Bcl2、Bax和血管内皮生长因子受体（VEGFR）的表达。综上所述，MOL提高了窝产仔数和抗氧化能力，降低了精子畸形率和Bax的表达。因此，MOL可作为一种功能性饲料添加剂用于提高动物繁殖性能。
