Veliev Farida, Briançon-Marjollet Anne, Bouchiat Vincent, Delacour Cécile
Université Grenoble Alpes, CNRS, Inst. NEEL, F-38000 Grenoble, France.
Université Grenoble Alpes, INSERM U1042, HP2, F-38041 Grenoble, France.
Biomaterials. 2016 Apr;86:33-41. doi: 10.1016/j.biomaterials.2016.01.042. Epub 2016 Feb 2.
Due to its outstanding mechanical and electrical properties as well as chemical inertness, graphene has attracted a growing interest in the field of bioelectric interfacing. Herein, we investigate the suitability of pristine, i.e. without a cell adhesive coating, chemical vapor deposition (CVD) grown monolayer graphene to act as a platform for neuronal growth. We study the development of primary hippocampal neurons grown on bare graphene (transferred on glass coverslip) for up to 5 days and show that pristine graphene significantly improves the neurons adhesion and outgrowth at the early stage of culture (1-2 days in vitro). At the later development stage, neurons grown on coating free graphene (untreated with poly-L-lysine) show remarkably well developed neuritic architecture similar to those cultured on conventional poly-L-lysine coated glass coverslips. This exceptional possibility to bypass the adhesive coating allows a direct electrical contact of graphene to the cells and reveals its great potential for chronic medical implants and tissue engineering. Moreover, regarding the controversial results obtained on the neuronal affinity of pristine graphene and its ability to support neuronal growth without the need of polymer or protein coating, we found that the crystallinity of CVD grown graphene plays an important role in neuronal attachment, outgrowth and axonal specification. In particular, we show that the decreasing crystalline quality of graphene tunes the neuronal affinity from highly adhesive to fully repellent.
由于其出色的机械和电学性能以及化学惰性，石墨烯在生物电接口领域引起了越来越多的关注。在此，我们研究了原始的（即没有细胞粘附涂层）化学气相沉积（CVD）生长的单层石墨烯作为神经元生长平台的适用性。我们研究了在裸露的石墨烯（转移到玻璃盖玻片上）上生长长达5天的原代海马神经元的发育情况，并表明原始石墨烯在培养早期（体外1-2天）显著改善了神经元的粘附和生长。在后期发育阶段，在无涂层石墨烯（未用聚-L-赖氨酸处理）上生长的神经元显示出与在传统聚-L-赖氨酸涂层玻璃盖玻片上培养的神经元相似的非常发达的神经突结构。这种绕过粘附涂层的特殊可能性使得石墨烯能够与细胞直接进行电接触，并揭示了其在慢性医疗植入物和组织工程中的巨大潜力。此外，鉴于在原始石墨烯的神经元亲和力及其在无需聚合物或蛋白质涂层的情况下支持神经元生长的能力方面获得的有争议的结果，我们发现CVD生长的石墨烯的结晶度在神经元附着、生长和轴突特化中起着重要作用。特别是，我们表明石墨烯结晶质量的下降将神经元亲和力从高粘附性调节为完全排斥性。
