Tronser Tina, Popova Anna A, Levkin Pavel A
Karlsruhe Institute of Technology (KIT), Institute of Toxicology and Genetics (ITG), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Karlsruhe Institute of Technology (KIT), Institute of Toxicology and Genetics (ITG), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany; Karlsruhe Institute of Technology (KIT), Institute of Organic Chemistry, 76131 Karlsruhe, Germany.
Curr Opin Biotechnol. 2017 Aug;46:141-149. doi: 10.1016/j.copbio.2017.03.005. Epub 2017 Apr 4.
Over the past decades stem cells have gained great interest in clinical research, tissue engineering and regenerative medicine, due to their ability of self-renewal and potential to differentiate into the various cell types of the organism. The long-term maintenance of these unique properties and the control of stem cell differentiation in vitro, however, remains challenging, thus limiting their applicability in these fields. High-throughput screening (HTS) of stem cells is widely used by the researchers in order to gain more insight in the underlying mechanisms of stem cell fate as well as identifying compounds and factors maintaining stemness. However, limited availability and expandability of stem cells restricts the use of microtiter plates for HTS of stem cells emitting the urge for miniaturized platforms. This review highlights recent advances in the development of miniaturized platforms for HTS of stem cells and presents novel designs of miniaturized HTS systems.
在过去几十年中，干细胞因其自我更新能力以及分化为机体各种细胞类型的潜力，在临床研究、组织工程和再生医学领域引起了极大关注。然而，在体外长期维持这些独特特性并控制干细胞分化仍然具有挑战性，从而限制了它们在这些领域的应用。研究人员广泛使用干细胞的高通量筛选（HTS），以便更深入地了解干细胞命运的潜在机制，并识别维持干性的化合物和因子。然而，干细胞有限的可用性和可扩展性限制了微孔板在干细胞高通量筛选中的使用，这促使人们需要小型化平台。本综述重点介绍了干细胞高通量筛选小型化平台开发的最新进展，并展示了小型化高通量筛选系统的新颖设计。
