Du Yue, Herath Sahan C B, Wang Qing-guo, Wang Dong-an, Asada H Harry, Chen Peter C Y
Department of Mechanical Engineering, National University of Singapore, Singapore.
BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology Program, Singapore.
Sci Rep. 2016 Feb 23;6:21362. doi: 10.1038/srep21362.
We studied the three-dimensional cell-extracellular matrix interactions of endothelial cells that form multicellular structures called sprouts. We analyzed the data collected in-situ from angiogenic sprouting experiments and identified the differentiated interaction behavior exhibited by the tip and stalk cells. Moreover, our analysis of the tip cell lamellipodia revealed the diversity in their interaction behavior under certain conditions (e.g., when the heading of a sprout is switched approximately between the long-axis direction of two different lamellipodia). This study marks the first time that new characteristics of such interactions have been identified with shape changes in the sprouts and the associated rearrangements of collagen fibers. Clear illustrations of such changes are depicted in three-dimensional views.
我们研究了形成称为芽的多细胞结构的内皮细胞的三维细胞-细胞外基质相互作用。我们分析了从血管生成芽生实验原位收集的数据，并确定了尖端细胞和茎细胞表现出的不同相互作用行为。此外，我们对尖端细胞片足的分析揭示了它们在某些条件下（例如，当一个芽的方向大约在两个不同片足的长轴方向之间切换时）相互作用行为的多样性。这项研究首次通过芽的形状变化和相关胶原纤维的重排确定了这种相互作用的新特征。这些变化的清晰图示以三维视图呈现。
