Mustafa Ibrahim, Al Marwani Asma, Mamdouh Nasr Khuloud, Abdulla Kano Noora, Hadwan Tameem
Health Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar.
Hematology Department, Hamad General Hospital, Hamad Medical Corporation, Doha, Qatar.
Biomed Res Int. 2016;2016:4529434. doi: 10.1155/2016/4529434. Epub 2016 Jan 21.
Usually packed red blood cells (pRBCs) require specific conditions in storage procedures to ensure the maximum shelf life of up to 42 days in 2-6°C. However, molecular and biochemical consequences can affect the stored blood cells; these changes are collectively labeled as storage lesions. In this study, the effect of prolonged storage was assessed through investigating morphological changes and evaluating oxidative stress. Samples from leukodepleted pRBC in SAGM stored at 4°C for 42 days were withdrawn aseptically on day 0, day 14, day 28, and day 42. Morphological changes were observed using scanning electron microscopy and correlated with osmotic fragility and hematocrit. Oxidative injury was studied through assessing MDA level as a marker for lipid peroxidation. Osmotic fragility test showed that extended storage time caused increase in the osmotic fragility. The hematocrit increased by 6.6% from day 0 to day 42. The last 2 weeks show alteration in the morphology with the appearance of echinocytes and spherocytes. Storage lesions and morphological alterations appeared to affect RBCs during the storage period. Further studies should be performed to develop strategies that will aid in the improvement of stored pRBC quality and efficacy.
通常情况下，浓缩红细胞（pRBCs）在储存过程中需要特定条件，以确保在2-6°C下最长保质期可达42天。然而，分子和生化方面的后果会影响储存的血细胞；这些变化统称为储存损伤。在本研究中，通过研究形态变化和评估氧化应激来评估延长储存的影响。从在4°C下储存于SAGM中的去白细胞pRBC中，在第0天、第14天、第28天和第42天无菌抽取样本。使用扫描电子显微镜观察形态变化，并与渗透脆性和血细胞比容相关联。通过评估丙二醛（MDA）水平作为脂质过氧化的标志物来研究氧化损伤。渗透脆性试验表明，延长储存时间会导致渗透脆性增加。从第0天到第42天，血细胞比容增加了6.6%。在最后两周观察到形态改变，出现了棘形红细胞和球形红细胞。储存损伤和形态改变似乎在储存期间影响红细胞。应进行进一步研究以制定有助于提高储存pRBC质量和功效的策略。
