Yamazaki Yohei, Meirelles Pedro Milet, Mino Sayaka, Suda Wataru, Oshima Kenshiro, Hattori Masahira, Thompson Fabiano L, Sakai Yuichi, Sawabe Toko, Sawabe Tomoo
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Institute of Biology, SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Sci Rep. 2016 Feb 24;6:21631. doi: 10.1038/srep21631.
Gut microbiome shapes various aspects of a host's physiology, but these functions in aquatic animal hosts have yet to be fully investigated. The sea cucumber Apostichopus japonicus Selenka is one such example. The large growth gap in their body size has delayed the development of intensive aquaculture, nevertheless the species is in urgent need of conservation. To understand possible contributions of the gut microbiome to its host's growth, individual fecal microbiome comparisons were performed. High-throughput 16S rRNA sequencing revealed significantly different microbiota in larger and smaller individuals; Rhodobacterales in particular was the most significantly abundant bacterial group in the larger specimens. Further shotgun metagenome of representative samples revealed a significant abundance of microbiome retaining polyhydroxybutyrate (PHB) metabolism genes in the largest individual. The PHB metabolism reads were potentially derived from Rhodobacterales. These results imply a possible link between microbial PHB producers and potential growth promotion in Deuterostomia marine invertebrates.
肠道微生物群塑造宿主生理的各个方面，但这些功能在水生动物宿主中的作用尚未得到充分研究。海参刺参就是这样一个例子。它们体型上的巨大生长差距延缓了集约化水产养殖的发展，不过该物种迫切需要保护。为了了解肠道微生物群对其宿主生长的可能贡献，进行了个体粪便微生物群比较。高通量16S rRNA测序显示，大小个体中的微生物群存在显著差异；特别是红杆菌目在较大个体中是最显著丰富的细菌类群。对代表性样本进一步进行鸟枪法宏基因组测序发现，最大个体中保留多羟基丁酸（PHB）代谢基因的微生物群显著丰富。PHB代谢读数可能来自红杆菌目。这些结果暗示了微生物PHB生产者与后口动物海洋无脊椎动物潜在生长促进之间的可能联系。
