Nawrotek Katarzyna, Mąkiewicz Mariusz, Zawadzki Dawid
Department of Environmental Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland.
Polymers (Basel). 2021 Mar 3;13(5):775. doi: 10.3390/polym13050775.
Major efforts for the advancement of tubular-shaped implant fabrication focused recently on the development of 3D printing methods that can enable the fabrication of complete devices in a single printing process. However, the main limitation of these solutions is the use of non-biocompatible polymers. Therefore, a new technology for obtaining hybrid implants that employ polymer extrusion and electrophoretic deposition is applied. The fabricated structures are made of two layers: polycaprolactone skeleton and chitosan-hydroxyapatite electrodeposit. Both of them can be functionalized by incorporation of mechanical or biological cues that favor ingrowth, guidance, and correct targeting of axons. The electrodeposition process is conducted at different voltages in order to determine the influence of this process on the structural, chemical, and mechanical properties of implants. In addition, changes in mechanical properties of implants during their incubation in phosphate-buffered solution (pH 7.4) at 37 °C up to 28 days are examined. The presented technology, being low-cost and relatively simple, shall find a broad scope of applications in customized nerve tissue engineering.
近期，管状植入物制造技术的主要发展方向集中在3D打印方法的开发上，这种方法能够在单一打印过程中制造出完整的装置。然而，这些解决方案的主要局限性在于使用了非生物相容性聚合物。因此，一种采用聚合物挤出和电泳沉积来制备混合植入物的新技术被应用。制造出的结构由两层组成：聚己内酯骨架和壳聚糖-羟基磷灰石电沉积物。通过掺入有利于轴突向内生长、引导和正确靶向的机械或生物学信号，这两层都可以实现功能化。为了确定该过程对植入物的结构、化学和机械性能的影响，电泳沉积过程在不同电压下进行。此外，还研究了植入物在37°C的磷酸盐缓冲溶液（pH 7.4）中孵育长达28天期间其机械性能的变化。所提出的技术成本低且相对简单，将在定制神经组织工程中找到广泛的应用范围。
