（13）C-甘露醇作为测量肠道通透性的新型生物标志物。
(13) C mannitol as a novel biomarker for measurement of intestinal permeability.
作者信息
Grover M, Camilleri M, Hines J, Burton D, Ryks M, Wadhwa A, Sundt W, Dyer R, Singh R J
机构信息
Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
出版信息
Neurogastroenterol Motil. 2016 Jul;28(7):1114-9. doi: 10.1111/nmo.12802. Epub 2016 Feb 23.
BACKGROUND
Gastrointestinal (GI) and non-GI disorders are associated with altered intestinal permeability, which can be measured in vivo by urinary excretion after oral lactulose and mannitol ingestion. Inadvertent dietary consumption of (12) Carbon ((12) C, regular) mannitol in food or from other sources may interfere with the test's interpretation. (13) Carbon ((13) C) constitutes 1% of carbon in nature and (13) C mannitol is a stable isotope. Our aim was to determine the performance of (13) C mannitol for measurement of intestinal permeability.
METHODS
Ten healthy volunteers underwent intestinal permeability assay using coadministered (12) C mannitol, (13) C mannitol and lactulose, followed by timed urine collections. Urinary sugar concentrations were measured using tandem high performance liquid chromatography-mass spectrometry.
KEY RESULTS
We found that (13) C mannitol can be distinguishable from (12) C mannitol on tandem mass spectrometry. In addition, (13) C mannitol had ~20-fold lower baseline contamination compared to (12) C mannitol. We describe here the (13) C mannitol assay method for the measurement of intestinal permeability.
CONCLUSIONS & INFERENCES: In conclusion, (13) C mannitol is superior to (12) C mannitol for measurement of intestinal permeability. It avoids issues with baseline contamination and erratic excretions during the testing period.
背景
胃肠道（GI）疾病和非胃肠道疾病都与肠道通透性改变有关，口服乳果糖和甘露醇后，可通过尿液排泄在体内测量肠道通透性。食物中或其他来源意外摄入的（12）碳（（12）C，常规）甘露醇可能会干扰测试结果的解读。（13）碳（（13）C）占自然界碳的1%，（13）C甘露醇是一种稳定同位素。我们的目的是确定（13）C甘露醇在测量肠道通透性方面的性能。
方法
10名健康志愿者接受了肠道通透性检测，同时服用（12）C甘露醇、（13）C甘露醇和乳果糖，随后定时收集尿液。使用串联高效液相色谱-质谱法测量尿糖浓度。
主要结果
我们发现，在串联质谱上（13）C甘露醇可与（12）C甘露醇区分开来。此外，与（12）C甘露醇相比，（13）C甘露醇的基线污染低约20倍。我们在此描述了用于测量肠道通透性的（13）C甘露醇检测方法。
结论与推论
总之，在测量肠道通透性方面，（13）C甘露醇优于（12）C甘露醇。它避免了测试期间基线污染和排泄不稳定的问题。
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