Petersen Janni, Russell Paul
Flinders University, Flinders Centre for Innovation in Cancer, School of Medicine, FMST, Bedford Park, Adelaide, South Australia 5042, Australia;
Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037.
Cold Spring Harb Protoc. 2016 Mar 1;2016(3):pdb.top079764. doi: 10.1101/pdb.top079764.
Here, we summarize the composition and uses of Schizosaccharomyces pombe media and discuss key issues for consideration in the generation of S. pombe cultures. We discuss the concept of "culture memory," in which the growth state and stress experienced by a strain during storage, propagation, and starter culture preparation can alter experimental outcomes at later stages. We also describe the triggers that are widely used to manipulate signaling through the environment sensing pathways.
在此，我们总结了粟酒裂殖酵母培养基的组成和用途，并讨论了粟酒裂殖酵母培养物生成过程中需要考虑的关键问题。我们探讨了“培养记忆”的概念，即菌株在储存、传代和起始培养物制备过程中经历的生长状态和压力会改变后期的实验结果。我们还描述了广泛用于通过环境感应途径操纵信号传导的触发因素。
