Bai Jing, Lu Yuanyuan, Xu Ya-ming, Zhang Wei, Chen Ming, Lin Min, Gunatilaka A A Leslie, Xu Yuquan, Molnár István
Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, Beijing 100081, P. R. China.
Natural Products Center, School of Natural Resources and the Environment, The University of Arizona , 250 East Valencia Road, Tucson, Arizona 85706, United States.
Org Lett. 2016 Mar 18;18(6):1262-5. doi: 10.1021/acs.orglett.6b00110. Epub 2016 Mar 2.
Two disparate polyketide families, the benzenediol lactones and the azaphilones, are produced by fungi using iterative polyketide synthase (iPKS) enzymes consisting of collaborating partner subunits. Exploitation of this common biosynthetic logic using iPKS subunit shuffling allowed the diversity-oriented combinatorial biosynthesis of unprecedented polyketide scaffolds new to nature, bearing structural motifs from both of these orthogonal natural product families. Starter unit acyltransferase domain replacements proved necessary but not sufficient to guarantee communication between iPKS subunits.
两个不同的聚酮化合物家族，即苯二醇内酯和氮杂蒽酮，是由真菌利用由协作伙伴亚基组成的迭代聚酮合酶（iPKS）酶产生的。利用iPKS亚基改组来开发这种常见的生物合成逻辑，实现了自然界中前所未有的聚酮化合物支架的多样性导向组合生物合成，这些支架带有来自这两个正交天然产物家族的结构基序。起始单元酰基转移酶结构域的替换被证明是必要的，但不足以保证iPKS亚基之间的通讯。
