从人淋巴细胞中提取的γ（免疫）干扰素信使核糖核酸的部分特性分析
Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.
作者信息
Taniguchi T, Pang R H, Yip Y K, Henriksen D, Vilcek J
出版信息
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3469-72. doi: 10.1073/pnas.78.6.3469.
Abstract
gamma (immune) interferon (IFN-gamma) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-gamma yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-gamma production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-gamma. Sucrose density gradient centrifugation analysis showed that IFN-gamma mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.
摘要
γ（免疫）干扰素（IFN-γ）是通过用佛波酯（12-O-十四酰佛波醇13-乙酸酯，TPA）和T细胞有丝分裂原植物血凝素（PHA）联合处理新鲜人淋巴细胞培养物而诱导产生的。与单独用PHA诱导获得的IFN-γ产量相比，加入TPA导致IFN-γ产量显著提高。从用TPA和PHA诱导的单核细胞中分离出含poly(A)的mRNA。将这种mRNA制剂注射到非洲爪蟾卵母细胞中，产生了具有人IFN-γ特征性质的IFN活性。蔗糖密度梯度离心分析表明，IFN-γ mRNA在15 S处沉降，表明它总共含有约1400个核苷酸。
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