Houghton M, Jackson I J, Porter A G, Doel S M, Catlin G H, Barber C, Carey N H
Nucleic Acids Res. 1981 Jan 24;9(2):247-66. doi: 10.1093/nar/9.2.247.
Experiments in which immobilised restriction fragments of genomic DNA were hybridised with a cloned human fibroblast interferon cDNA indicate that the homologous chromosomal genes exist in only one basic arrangement. This is in marked contrast to recent studies by Nagata et al. (1) showing that there are at least eight gene arrangements for human leukocyte interferon. Having isolated a chromosomal human fibroblast interferon gene from a gene bank, we conclude from nucleotide sequencing studies that there is a complete absence of introns within the RNA-coding region. In view of a similar observation recently made for a human leukocyte interferon gene (1), it would appear as if interferon genes in general are unlike the vast majority of eukaryote genes in this respect.
将固定的基因组DNA限制片段与克隆的人成纤维细胞干扰素cDNA进行杂交的实验表明，同源染色体基因仅以一种基本排列存在。这与Nagata等人最近的研究（1）形成了显著对比，后者表明人白细胞干扰素至少有八种基因排列。从基因库中分离出一个染色体人成纤维细胞干扰素基因后，我们通过核苷酸测序研究得出结论，RNA编码区内完全没有内含子。鉴于最近对人白细胞干扰素基因也有类似的观察结果（1），在这方面，干扰素基因似乎与绝大多数真核生物基因不同。
