Pang R H, Hayes T G, Vilcek J
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5341-5. doi: 10.1073/pnas.77.9.5341.
Human F and Le interferon can be clearly distinguished on the basis of different antigenic properties and host range. After inoculation with Newcastle disease virus (NDV), GM-258 fibroblasts produced Le as well as F interferon; in contrast, only F interferon was detectable after stimulation with poly(I) . poly(C). Polyadenylylated mRNA isolated from fibroblasts induced with poly(I) . poly(C) or NDV was injected into Xenopus laevis oocytes and the interferon activities thus produced were analyzed. Only F interferon production was demonstrable in oocytes injected with mRNA from cells induced with poly(I) . poly(C), whereas both F and Le interferons were made in oocytes injected with mRNA from NDV-induced cultures. The time course of accumulation of F and Le interferon mRNAs in NDV-induced cells corresponded to the kinetics of F and Le interferon synthesis in intact cells. The ratio of F and Le interferons made in oocytes was similar to that observed in intact GM-258 cells. F and Le interferon mRNA activities isolated from GM-258 cells could not be separated by sucrose density gradient centrifugation. However, the profile of F mRNA activity was more heterogeneous and its peak sedimented somewhat more slowly than that of Le interferon mRNA. These results suggest that the varying ratios of F and Le interferon synthesis in different cells after different modes of stimulation are determined at the level of mRNA. The induction mechanisms of F and Le interferon mRNA synthesis appear to be closely related but not identical.
人F干扰素和Le干扰素可根据不同的抗原特性和宿主范围明确区分。用新城疫病毒（NDV）接种后，GM - 258成纤维细胞产生Le干扰素和F干扰素；相反，用聚肌苷酸-聚胞苷酸（poly(I)·poly(C)）刺激后仅可检测到F干扰素。将从用poly(I)·poly(C)或NDV诱导的成纤维细胞中分离出的多聚腺苷酸化mRNA注射到非洲爪蟾卵母细胞中，并分析由此产生的干扰素活性。在用poly(I)·poly(C)诱导的细胞的mRNA注射的卵母细胞中仅可证明有F干扰素产生，而在用NDV诱导培养物的mRNA注射的卵母细胞中F干扰素和Le干扰素均有产生。在NDV诱导的细胞中F干扰素和Le干扰素mRNA积累的时间进程与完整细胞中F干扰素和Le干扰素合成的动力学一致。在卵母细胞中产生的F干扰素和Le干扰素的比例与在完整的GM - 258细胞中观察到的相似。从GM - 258细胞中分离出的F干扰素和Le干扰素mRNA活性不能通过蔗糖密度梯度离心分离。然而，F mRNA活性的分布更不均一，其峰值沉降速度比Le干扰素mRNA略慢。这些结果表明，在不同刺激模式后不同细胞中F干扰素和Le干扰素合成比例的变化是在mRNA水平上决定的。F干扰素和Le干扰素mRNA合成的诱导机制似乎密切相关但并不相同。
