Maeda S, McCandliss R, Gross M, Sloma A, Familletti P C, Tabor J M, Evinger M, Levy W P, Pestka S
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7010-3. doi: 10.1073/pnas.77.12.7010.
Bacterial plasmids containing human leukocyte interferon sequences were constructed and identified. Identification was confirmed by correspondence of the nucleotide sequence with out amino acid sequence of human leukocyte interferon. The finding of bacterial recombinants containing distinct leukocyte interferon sequences is consistent with our purification of different leukocyte interferon species. We conclude that what has been designated human leukocyte interferon is, indeed, a class of homologous proteins. Preliminary indications suggest that their diversity appears to be represented by individual genomic equivalents. Each of the individual species exhibits characteristic activities. The structural modulation of these biological activities has immense significance for understanding the natural role of the interferons and for refining and developing their ultimate therapeutic potential.
构建并鉴定了含有人类白细胞干扰素序列的细菌质粒。通过核苷酸序列与人类白细胞干扰素氨基酸序列的对应关系证实了鉴定结果。含有不同白细胞干扰素序列的细菌重组体的发现与我们对不同白细胞干扰素种类的纯化结果一致。我们得出结论，所谓的人类白细胞干扰素确实是一类同源蛋白。初步迹象表明，它们的多样性似乎由个体基因组等价物来体现。每个个体种类都表现出特征性活性。这些生物活性的结构调节对于理解干扰素的天然作用以及完善和开发它们最终的治疗潜力具有巨大意义。
